Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome stability in different mouse embryonic stem cells [exome-seq]

ABSTRACT: Genome stability in different mouse embryonic stem cells [exome-seq]

PROVIDER: PRJNA663162 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12630500_1.fastq.gz	Fastqsanger.gz
	SRR12630500_2.fastq.gz	Fastqsanger.gz
	SRR12630501_1.fastq.gz	Fastqsanger.gz
	SRR12630501_2.fastq.gz	Fastqsanger.gz
	SRR12630502_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 42

Similar Datasets

Genome stability in different mouse embryonic stem cells

Project description:Genome stability in different mouse embryonic stem cells

| PRJNA663160 | ENA

Genome stability in different mouse embryonic stem cells super

Project description:Genome stability in different mouse embryonic stem cells super

| PRJNA663159 | ENA

Genome stability in different mouse embryonic stem cells [exome-seq]

Project description:Naïve mouse ESCs exhibit full term developmental competence thus hold great potential in regenerative medicine. Maintaining genome stability is essential for potential application of ESCs in stem cell therapy. While ESC potency fluctuate with retrovirus activity, it is unclear whether long-term cultures affect retrotransposition and genomic stability. We compared retrotransposons in naïve ESCs using various approaches. We show that feeder-serum based culture conditions and small molecule LCDM maintain appropriate expression of retrovirus activity following long-term cultures, whereas cultures under 2iLif and a2i result in aberrant upregulation of retrotransposons. This leads to high frequent retrotransposition. Moreover, naïve ESCs on feeder-serum based culture conditions and small molecule LCDM still generate complete ESC pups by TEC assay, functional test of pluripotency, following long-term cultures, despite that all culture conditions maintain high expression levels of pluripotent genes such as Oct4, Nanog, Klf4 and Sox2. Meanwhile, long telomeres are maintained in ESCs under cultures in Feeder-serum and LCDM conditions but telomeres shortened in other conditions with increasing passages. These data provide insights into retrotransposition, genomic stability and pluripotency of naïve ESCs.

2023-09-12 | GSE157875 | GEO

Genome stability in different mouse embryonic stem cells [ATAC-seq]

Project description:Genome stability in different mouse embryonic stem cells [ATAC-seq]

| PRJNA663161 | ENA

Genome stability in different mouse embryonic stem cells

2023-09-12 | GSE157874 | GEO

Genome stability in different mouse embryonic stem cells [ATAC-seq]

2023-09-12 | GSE157873 | GEO

Whole exome sequencing in SMRad51 and Control primary mouse embryonic fibroblasts

Project description:Homologous recombination (HR) is a Rad51-mediated evolutionary conserved process that plays a unique role in genome plasticity, controlling the balance genetic stability/diversity. In this study we compared the induction of genetic modifications by whole exome sequencing in primary mouse embryonic fibroblasts after the expression of a dominant negative form of Rad (SMRad51). We used a transgenic mouse embryonic fibroblasts in which SMRad51 expression was induced under doxycycline (Dox) control. We compared SMRad51 primary mouse embryonic fibroblasts treated with Dox (transgene expression induced) with control mouse embryonic fibroblasts treated with Dox.

2019-12-29 | E-MTAB-8625 | biostudies-arrayexpress

Biological Insights from the genome Sequences of Human Embryonic Stem Cell Lines

Project description:<p>Human embryonic stem cells (hESCs) are a powerful tool for the study of human development and can form the basis of cellular disease models or therapies. However, the genetic make-up and stability of hESCs has not been systematically studied at a genome-wide level with single nucleotide resolution. We therefore sequenced the whole exomes of widely available hESCs. The data generated has provided new insights about the nature of acquired variation and the genomic integrity of the cell lines. We anticipate that this online resource will enable investigators to access raw sequencing data in order to interrogate cell lines for different disease and trait-associated genetic variants.</p>

| phs001343 | dbGaP