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RNA-seq of T in PBMC and TILs

ABSTRACT: RNA-seq of T in PBMC and TILs

PROVIDER: PRJNA663398 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12639948_1.fastq.gz	Fastqsanger.gz
	SRR12639948_2.fastq.gz	Fastqsanger.gz
	SRR12639949_1.fastq.gz	Fastqsanger.gz
	SRR12639949_2.fastq.gz	Fastqsanger.gz

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RNA-seq of T in PBMC and TILs

Project description:Purpose: Transcriptome profiling (RNA-seq) was used to compare the expression differences between T in PBMC and TILs Methods:Paired TILs and T in PBMC cells were sorted from fresh patient blood and tumor samples. Then the mRNA were sequeced using xTen platform. The sequence reads that passed quality filters were analyzed. Results: by extract mRNA from tumor infiltrated T cells from ovarian cancer patient sample and T cells from patient bllod samples, we compared the different expressed genes from TIL and T in blood. Hierarchical clustering of differentially expressed genes uncovered several as yet uncharacterized genes that may contribute to T cell exhausetion. Conclusions: We conclude that T in PBMC and TILs share different transcriptome characterization.

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