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Single-cell RNA-sequencing of mouse and human lung Lin–EpCAM+ cells

ABSTRACT: Single-cell RNA-sequencing of mouse and human lung Lin–EpCAM+ cells

PROVIDER: PRJNA663598 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12645700.fastq.gz	Fastqsanger.gz
	SRR12645800.fastq.gz	Fastqsanger.gz
	SRR12645701.fastq.gz	Fastqsanger.gz
	SRR12645801.fastq.gz	Fastqsanger.gz
	SRR12645702.fastq.gz	Fastqsanger.gz

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Single-cell RNA-sequencing of human lung Lin–EpCAM+ cells

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Single-cell RNA-sequencing of human lung Lin–EpCAM+ cells

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Single-cell RNA-sequencing of mouse and human lung Lin–EpCAM+ cells

Project description:This SuperSeries is composed of the SubSeries listed below. These datasets include RNA transcriptomics of bleomycin-injured mouse lungs, day 0, day 4 and day 14; and corresponding controls; as well as RNA transcriptomics of lungs from six healthy donors and six patients with IPF.

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Single cell RNA-sequencing on healthy and IPF lung mesenchymal cells.

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Project description:Epithelial cell homeostasis and renewal in the lung requires the supportive signals from surrounding mesenchymal cells. Single cell RNA-seq analysis was used to examine the gene expression levels of several receptors on the Sftpc+ alveolar type II stem cells. Single cell RNA-seq revealed Ghr, Cxcr2, Cxcr4, and Igf1r expressing cells were all Sftpc+ cells in the normal mouse lung.

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Immune evasion in lung metastasis of leiomyosarcoma: upregulation of EPCAM inhibits CD8⁺ T cell infiltration

Project description:Background: Leiomyosarcomas are among the most common histological types of soft tissue sarcoma (STS), with no effective treatment available for advanced patients. Lung metastasis, the most common site of distant metastasis, is the primary prognostic factor. We analysed the immune environment targeting lung metastasis of STS to explore new targets for immunotherapy. Methods: We analysed the immune environment of primary and lung metastases in 38 patients with STS using immunohistochemistry. Next, we performed gene expression analyses on primary and lung metastatic tissues from six patients with leiomyosarcoma. Using human leiomyosarcoma cell lines, the effects of the identified genes on immune cells were assessed in vitro. Results: Immunohistochemistry showed a significant decrease in CD8⁺ cells in the lung metastases of leiomyosarcoma. Among the genes upregulated in lung metastases, epithelial cellular adhesion molecule (EPCAM) showed the strongest negative correlation with the number of CD8⁺ cells. Transwell assay results showed that the migration of CD8⁺ T cells was significantly increased in the conditioned media obtained after inhibition or knock down of EPCAM. Conclusions: EPCAM was upregulated in lung metastases of leiomyosarcoma, suggesting inhibition of CD8⁺ T cell migration. Our findings suggest that EPCAM could serve as a potential novel therapeutic target for leiomyosarcoma.

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Next Generation Sequencing of LSK EpCAM+ and EpCAM- of Zeb1-/-

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2012-08-08 | GSE31313 | GEO

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