Project description:To survey the proteomic differences between WT and Slc37a2 knockout osteoclasts at the whole cell and secretory lysosome levels, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed’ with SPIONs to encourage uptake into endosomes and then ‘chased’ into secretory lysosomes upon the convergence of SPION-loaded endosomes with lysosomes and secretory pathways. Following the ‘pulse-chase’, osteoclasts were homogenized, SPION-loaded organelles captured-from post-nuclear supernatants using magnetic columns, and enriched organelles as well as homogenates eluted and processed for 1D in-gel digestion and mass spectrometry. The samples presented here correspond to the proteome of WT and Slc37a2 knockout homogenates with secretory lysosome proteomes have been shared in another submission.

2023-01-25 | PXD037910 | Pride

Lung gene expression analysis of WT, Nlrp3-, Asc-deficient mice in Streptococcus pneumoniae D39 and ATCC6303 infection

Project description:Streptococcus (S.) pneumoniae is the most frequently isolated causative pathogen community-acquired pneumonia, a leading cause of mortality worldwide. We investigated the role of the inflammasome sensor NLRP3 and the inflammasome adapter ASC during S. pneumoniae pneumonia. Detailed analysis of the early inflammatory response in the lung by whole genome transcriptional profiling, we identified several mediators that were differentially expressed between Nlrp3-/- and Asc-/ - mice. WT, Nlrp3- and Asc-deficient mice were intranasally inocculated with Streptococcus pneumoniae D39 and ATCC6303 both at high and low dose. Lung homogenates were harvested and gene expression profiling was performed.

2014-11-27 | E-GEOD-42464 | biostudies-arrayexpress

Whole-genome gene expression profiles of non-tumorous human lung tissues

Project description:This SuperSeries is composed of the following subset Series: GSE23352: Whole-genome gene expression profiles of non-tumorous human lung tissues: Laval set GSE23529: Whole-genome gene expression profiles of non-tumorous human lung tissues: UBC set GSE23545: Whole-genome gene expression profiles of non-tumorous human lung tissues: GRNG set Refer to individual Series

2012-06-10 | E-GEOD-23546 | biostudies-arrayexpress

Whole lung gene expression from naïve Bpifb1 WT and KO mice

Project description:We performed RNAsequencing on whole lung tissue from naïve Bpifb1 WT and KO mice.

2020-09-01 | GSE150452 | GEO

Lung gene expression

Project description:Lung gene expression

| PRJNA321196 | ENA

Whole lung gene expression in Tcrd-/- mice after Burkholderia thailandensis infection

Project description:Neutrophil recruitment appears critical to the pulmonary defense to B. pseudomallei. Therefore, we hypothesized that γδ T cells are critical regulators of pulmonary bacterial clearance given their proposed regulation of neutrophil recruitment to the lung. To investigate this hypothesis, we leveraged multiple in vivo models of pulmonary melioidosis to further understand the mechanisms by which γδ T cells regulate inflammation early in severe pulmonary infection. Included in this investigation, we assessed whole lung bulk RNA-sequencing data in mice with and without γδ T cells after infection with Burkholderia thailandensis.

2024-06-28 | GSE254868 | GEO

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