Project description:Parental and BXD mouse lines were received from Jackson Laboratory and University of Tennessee Health Science Center. All animals were housed at UTHSC before sacrifice. Mice were killed by cervical dislocation and whole lungs including blood were removed and placed in RNAlater. Total RNA was extracted from the lungs. RNA from two to five animals per strain were pooled and used for gene expression analysis. Animals used in this study were between 49 and 93 days of age. All inbred strains were profiled for both sexes, and for a given BXD strain either males or females were used. Gene expression was measured by microarrays. Several genes which showed a restricted expression to lung tissue were identified. Known QTL regions for respiratory infection susceptibility were investigated and several cis-eQTL genes were identified. Numerous cis- and trans-regulated transcripts and chromosomal intervals with strong regulatory activity were mapped.

2011-12-01 | E-MTAB-848 | biostudies-arrayexpress

Whole-genome expression data from normal FVB mouse lung tissue, transgenic cyclin E overexpressing (CEO) normal mouse lung tissue, and transgenic CEO lung adenocarcinomas

Project description:FVB mice were engineered to express wild-type human cyclin E under control of the human surfactant C promoter (CEO mice; Ma et al, PNAS 2007). These mice develop spontaneous lung tumors, which were shown to be adenocarcinoma by histological analysis. Here we compare whole-genome RNA expression levels between the tumors and normal lung of 4 CEO mice as well as 4 nontransgenic animals. RNA was isolated from the lungs of 4 FVB mice and adjacent normal and tumor tissue from 4 FVB transgenes harboring human surfactant protein C- driven wild-type human cyclin E, all 7-11 months in age. These samples were divided into 3 groups of four, and 12 independent hybridizations were performed for analysis with Affymetrix GeneChip Mouse 430 2.0 arrays.

2013-04-04 | E-GEOD-45744 | biostudies-arrayexpress

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