Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA663998 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12690255_1.fastq.gz | Fastqsanger.gz | |||
| SRR12690255_2.fastq.gz | Fastqsanger.gz | |||
| SRR12690256_1.fastq.gz | Fastqsanger.gz | |||
| SRR12690256_2.fastq.gz | Fastqsanger.gz | |||
| SRR12690257_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 16 |
Similar Datasets
Project description:Lung adenocarcinoma is heterogeneous and hierarchically organized, with a sub-population of stem-like cells (CSC) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here, we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLC).
2021-02-09 | PXD023981 |
Project description:In this study, we generated a quantitative lung tissue proteome dataset from lung adenocarcinoma and adjacent normal lung tissues by using iTRAQ labeling combined with 2D-LC-MS/MS. Based on pathway and network analyses, protein expression profiles released in the public Human Protein Atlas database, literature search and novelty, six candidates were selected for validation via immunohistochemistry staining and western blot. Additionally, the clinical and biological significance of two novel candidates, ERO1L and NARS, was further analyzed. Collectively, our results provide a useful biomarker dataset for lung adenocarcinoma diagnosis/prognosis and provide new insights into ERO1L- and NARS- mediated tumorigenesis.
2016-05-12 | PXD004077 | Pride
Project description:In this study, we generated a quantitative lung tissue proteome dataset from lung adenocarcinoma and adjacent normal lung tissues by using iTRAQ labeling combined with 2D-LC-MS/MS. Based on pathway and network analyses, protein expression profiles released in the public Human Protein Atlas database, literature search and novelty, six candidates were selected for validation via immunohistochemistry staining and western blot. Additionally, the clinical and biological significance of two novel candidates, ERO1L and NARS, was further analyzed. Collectively, our results provide a useful biomarker dataset for lung adenocarcinoma diagnosis/prognosis and provide new insights into ERO1L- and NARS- mediated tumorigenesis.
2022-03-05 | PXD004049 | Pride
Project description:A multi-omic method, which combined both proteomic and N-glycoproteomic analyses, was developed to analyze both the normal and cancerous bronchoalveolar lavage fluids (BALFs) of six lung adenocarcinoma patients for screening the new potential biomarkers of lung adenocarcinoma.
2024-10-08 | PXD056583 |
Project description:A multi-omic single-cell atlas of human gynecologic malignancies
| PRJNA699345 | ENA
Project description:A multi-omic single-cell atlas of human gynecologic malignancies
| PRJNA699347 | ENA
Project description:Stephen Paget first proposed, in 1889, that organ distribution of metastases is a non-random event, yet metastatic organotropism remains one of the greatest mysteries in cancer biology. Here, we demonstrate that exosomes released by lung-, liver- and brain-tropic tumor cells fuse preferentially with resident cells at their predicted destination, such as fibroblasts and epithelial cells in the lung, Kupffer cells in the liver, and endothelial cells in the brain. We found that exosome homing to organ-specific cell types prepares the pre-metastatic niche and that treatment with exosomes derived from lung tropic models can redirect metastasis to the lung. Proteomic profiling of exosomes revealed distinct integrin expression patterns associated with each organ-specific metastasis. Whereas exosomal integrins α6β4 and α6β1 were associated with lung metastasis, exosomal integrins αvβ5 and αvβ3 were linked with liver and brain metastases, respectively. Targeting α6β4 and αvβ5 integrins decreased exosome uptake and metastasis in the lung and liver, respectively. Importantly, we demonstrate that exosome uptake activates a cell-specific subset of S100 family genes, known to support cell migration and niche formation. Finally, our clinical data indicate that integrin-expression profiles in circulating plasma exosomes from cancer patients could be used to predict organ-specific metastasis. Education of human von Kupffer cells in vitro with human pancreatic cancer exosomes
2015-06-17 | E-GEOD-68919 | biostudies-arrayexpress
Project description:We conducted a multi-omics analysis including single-cell RNA transcriptome, single-cell T cell receptor (scTCR), whole exome sequencing and multiplex immunohistochemistry (mIHC) to decipher single cell atlas of precursor lung adenocarcinoma and mechanisms of diverse immune cells during precursor lesions development in hopes of digging out potential therapeutic targets.
2024-01-13 | GSE164789 | GEO
Project description:Exososmes, potent intercellular communicators, are supposed to contribute to metastasis formation, which we confirmed for exosomes of the metastatic rat pancreatic adenocarcinoma line BSp73ASML that promote metastatic settlement in lymph nodes and lung of poorly metastatic BSp73ASML cells with a selective CD44v4-v7 (BSp73ASML-CD44vkd) knockdown. To define the molecular pathway(s), whereby exosomes contribute to premetastatic niche preparation, we profiled mRNA miRNA of BSp73ASMLwt and BSp73ASML-CD44vkd- exosomes and evaluated the impact on potential target cells. BSp73ASML exosomes are recovered in the draining lymph node after subcutaneous injection. In vitro, they preferentially bind and are taken-up by lymph node stroma cells (LnStr) and lung fibroblasts (LuFb) that were chosen as exosome targets. BSp73ASMLwt and BSp73ASML-CD44kd exosomes contain a restricted repertoire of mRNA and miRNA, hwere the lattter differe significantly between the two lines and even more pronounced, exosomes derived thereof with a not yet explored dominance of tumor-suppressor miRNA in ASML-CD44kd cells and exosomes. Both, exosomal mRNA and miRNA are recovered in target cells and exosome-uptake is accompanied by significant changes in gene expression. We didn't observe a correlation between exosomal mRNA and changes in target cell mRNA or proteins. Instead transferred miRNA significantly affected target cell mRNA translation as demonstrated for selected, most abundant ASML exosomal miRNA besides others, miR-494 known target MAL (myelin and lymphocytes protein)/cadherin17, and miR-542-3p which targets TRAF/cadherin17. Furthermore, MMP transcription suggested to accompany cadherin17 dwon-regulation was upregulated in miR-494 or miR542-3p transfected or exosome co-cultured LnStr. Taken together, tumor exosomes target in vivo non-transformed cells in premetastatic organs. Exosome uptake induced altered target celll gene expression is strongly promoted by exosomal miRNA where we demonstrate for the first time that exosomes/exosomal miRNA from a metastasizing tumor line can modulate stroma cells from premetastatic organs. Endothelial cells lines were treated with pancreatic adenocarcinoma (AS) derived exosomes or pancreatic adenocarcinoma derived exosomes expressing tetraspanin 8. Total RNA was isolated and used to perform the Agilent gene expression microarrays. In this assay a replicate of endothelial cell lines treated with ASTspan8 were also included. Moreover, total RNA from both base line expression of endothelial cells and rat endothelial fibroblasts were also used to perfrom gene expression microarrays. RNA isolated from Rat endothelial fibroblasts treated with the exosomes derived from rat pancreatic adenocarcinoma and exosomes derived from rat pancreatic adenocarcinoma expressing tetraspanin8 were individually used to perfrom gene expression microarrays. RNA isolated from exosomes derived from rat pancreatic adenocarcinoma cell lines expressing tetraspanin were used to peform gene expresiion to see the base line expression. Another replicate were also used. RNA isolated from base line or control of rat pancreatic adenocarcinoma wild type cells and also base line RNA isolated from rat pancreatic adenocarcinoma cells lines where CD44 was knock-down.
2012-06-12 | E-GEOD-38655 | biostudies-arrayexpress
Project description:Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor prognosis of patients. Exosomes are involved in mediating cellular environment interactions. circRNAs are a class of non-coding RNAs (ncRNAs) broadly expressed in cells and exosomes. However, in lung adenocarcinoma tumor development, functions and regulatory mechanisms of exosomal circ-RNAs induced by hypoxia remain poorly understood.To further explore the differences in recombinant RNA expression in hypoxic and normoxic exosomes derived from lung adenocarcinoma cell line
2023-01-21 | GSE144053 | GEO