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Single-cell Transcriptomic Landscape of Human Blood Cells

ABSTRACT: Single-cell Transcriptomic Landscape of Human Blood Cells

PROVIDER: PRJNA664104 | ENA |

REPOSITORIES: ENA

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Single-cell transcriptomic landscape of human blood cells.

Project description:High throughput single-cell RNA-seq has been successfully implemented to dissect the cellular and molecular features underlying hematopoiesis. However, an elaborate and comprehensive transcriptome reference of the whole blood system is lacking. Here, we profiled the transcriptomes of 7551 human blood cells representing 32 immunophenotypic cell types, including hematopoietic stem cells, progenitors and mature blood cells derived from 21 healthy donors. With high sequencing depth and coverage, we constructed a single-cell transcriptional atlas of blood cells (ABC) on the basis of both protein-coding genes and long noncoding RNAs (lncRNAs), and showed a high consistence between them. Notably, putative lncRNAs and transcription factors regulating hematopoietic cell differentiation were identified. While common transcription factor regulatory networks were activated in neutrophils and monocytes, lymphoid cells dramatically changed their regulatory networks during differentiation. Furthermore, we showed a subset of nucleated erythrocytes actively expressing immune signals, suggesting the existence of erythroid precursors with immune functions. Finally, a web portal offering transcriptome browsing and blood cell type prediction has been established. Thus, our work provides a transcriptional map of human blood cells at single-cell resolution, thereby offering a comprehensive reference for the exploration of physiological and pathological hematopoiesis.

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Single-cell Transcriptomic Landscape of Human Blood Cells

Project description:We profiled the transcriptomes of 7,643 human blood cells covering 32 immunophenotypic cell types across 21 healthy donors. We constructed the single-cell transcriptional atlas of blood cells on the basis of protein-coding genes and long noncoding RNAs (lncRNAs) respectively, and showed a high consistence between them. Notably, lncRNAs and novel transcription factors regulating hematopoietic cell differentiation were identified.

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