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Platelet microRNA bioinformatics analysis in patients with hepatocellular carcinoma

ABSTRACT: Platelet microRNA bioinformatics analysis in patients with hepatocellular carcinoma

PROVIDER: PRJNA665508 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12707564_1.fastq.gz	Fastqsanger.gz
	SRR12707564_2.fastq.gz	Fastqsanger.gz
	SRR12707565_1.fastq.gz	Fastqsanger.gz
	SRR12707565_2.fastq.gz	Fastqsanger.gz
	SRR12707566_1.fastq.gz	Fastqsanger.gz

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Platelet microRNA bioinformatics analysis in patients with hepatocellular carcinoma

Project description:Purpose:To reveal platelet microRNAs（miRNAs） expression status and its potential clinical significance in patients with hepatocellular carcinoma(HCC) by bioinformatics analysis. Method: 5 patients (4 male, 1 female) with hepatocellular carcinoma, and 5 healthy individuals(3 male，2 female) as control from June 2019 to November 2019 were taken part in this cohort. The leukocyte—depleted platelets were prepared from the anticoagulated whole blood samples. The platelet RNA were extracted to construct miRNA libraries by using conventional gradient centrifugation, MACS and instructions of miRNeasy Mini Kit, performed data analysis and bioinformatics analysis after sequencing. Results: The DESeq2 package of R software was used to compare the gene expression level difference between the HCC group and the normal group. The cut-off criteria of differentially expressed miRNAs was |log2FC| >1 and p<0.05. Total 250 differentially expressed miRNAs were screened out, of which 111 miRNAs were down-regulated and 139 miRNAs were up-regulated. Gene Ontology(GO) analysis found that the differential miRNAs are involved in Positional Regulatory of Catabolic process, positional regulatory of cell catabolic process, negative regulation of phosphorylation, Wnt signaling pathway. Kyoto Encyclopedia of Genes and Genomes (KEGG)pathway analysis showed that platelet miRNAs are involved in a number of signal pathways, including FoxO signaling pathway, ubiquitin mediated proteolysis, axon guidance, autophagy-animal, TGF-beta signaling pathway. Conclusion:MiRNAs in platelets of patients with hepatocellular carcinoma were differentially expressed. Platelet miRNAs should be involved in the biological process of hepatocellular carcinoma, while more samples and molecular biological experiments are needed.

2021-09-21 | GSE158523 | GEO

microRNA profiling of human hepatocellular carcinoma

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Platelet microRNA expression profiles of acute coronary syndrome patients with high and low platelet reactivity

Project description:To explore the diverse platelet microRNA (miRNA) expression between high platelet reactivity (HPR) and low platelet reactivity (LPR) patients with acute coronary syndromes (ACS), we enrolled a cohort of ACS patients and performed miRNA expression profiling of platelets from four HPR and four LPR patients using human miRNA microarray system. VerifyNow P2Y12 assay was applied to indentify HPR and LPR. Venous blood was drawn from the patients and was centrifuged to prepare platelets. Among the candidate differentially expressed miRNAs, miR-15b expression was further confirmed to be lower in platelets of 22 HPR patients than 17 LPR by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). We enrolled a consecutive cohort of 290 ACS patients and assessed the platelet reactivity using VerifyNow P2Y12 assay. In this study, HPR was defined as M-bM-^IM-%300 platelet reactivity unit (PRU) while LPR <170 PRU. miRNA microarray analysis was performed in platelets of four HPR and four LPR patients with ACS.

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MicroRNA expression profile in human hepatocellular carcinoma

Project description:MicroRNA expression profile in human hepatocellular carcinoma

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microRNA profiling of human healthy liver and hepatocellular carcinoma

Project description:microRNA profiling of 10 human healthy liver and 9 human hepatocellular carcinoma tissues using bead-based technology

2012-07-22 | GSE31383 | GEO

mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

Project description:mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7 Keywords: Hepatocellular carcinoma, Expression array, microRNA

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