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MicroRNA profiling of human hepatocellular carcinoma

ABSTRACT: microRNA profiling of 89 human hepatocellular carcinoma tissues using bead-based technology. microRNA expression profiling was performed using freshly frozen hepatocellular carcinoma tissues obtained at the time of surgical treatment.

ORGANISM(S): Homo sapiens

SUBMITTER: Yujin Hoshida

PROVIDER: E-GEOD-20594 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA-based classification of hepatocellular carcinoma and oncogenic role of miR-517a.

Toffanin Sara S Hoshida Yujin Y Lachenmayer Anja A Villanueva Augusto A Cabellos Laia L Minguez Beatriz B Savic Radoslav R Ward Stephen C SC Thung Swan S Chiang Derek Y DY Alsinet Clara C Tovar Victoria V Roayaie Sasan S Schwartz Myron M Bruix Jordi J Waxman Samuel S Friedman Scott L SL Golub Todd T Mazzaferro Vincenzo V Llovet Josep M JM

Gastroenterology 20110213 5

<h4>Background & aims</h4>Hepatocellular carcinoma (HCC) is a heterogeneous tumor that develops via activation of multiple pathways and molecular alterations. It has been a challenge to identify molecular classes of HCC and design treatment strategies for each specific subtype. MicroRNAs (miRNAs) are involved in HCC pathogenesis, and their expression profiles have been used to classify cancers. We analyzed miRNA expression in human HCC samples to identify molecular subclasses and oncogenic miRNA ...[more]

PMID: 21324318

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microRNA profiling of human healthy liver and hepatocellular carcinoma

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2012-07-21 | E-GEOD-31383 | biostudies-arrayexpress

MicroRNA-Based Classification of Hepatocellular Carcinoma and Oncogenic Role of miR-517a

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microRNA expression in murine hematopoietic stem cells

Project description:MicroRNAs influence hematopoietic differentiation, but little is known about their effects on the stem cell state. Here, we report that the microRNA processing enzyme Dicer is essential for stem cell persistence in vivo and a specific microRNA, miR-125a controls the size of the stem cell population by regulating stem/progenitor cell (HSPC) apoptosis. Conditional deletion of Dicer revealed an absolute dependence for the multipotent HSPC population in a cell autonomous manner, with increased HSPC apoptosis in mutant animals. An evolutionarily conserved microRNA cluster containing miR-99b, let-7e and miR-125a was preferentially expressed in long term HSCs. miR-125a alone was capable of increasing the number of hematopoietic stem cells in vivo by more than eight fold. This was accomplished through a differentiation stage-specific reduction of apoptosis in immature hematopoietic progenitors, possibly through targeting multiple pro-apoptotic genes. Bak1 was directly down-regulated by miR-125a and expression of a 3’UTR-less Bak1 blocked miR-125a-induced hematopoietic expansion in vivo. These data demonstrate cell-state-specific regulation by microRNA and identify a unique microRNA functioning to regulate the stem cell pool size. Bone marrow populations were FACS-sorted and profiled using a bead-based profiling platform. Long-term HSCs, short-term HSCs, multipotent progenitors, Lin-Kit+Sca+ cells, Lin-Kit+Sca- cells, Lin-Kit-Sca+ cells, Lin- cells and unfractionated whole bone marrow cells were prepared for total RNA using TriZol (Invitrogen) in replicates. For rare populations, cells from multiple mice were pooled. To perform microRNA profiling, 60 ng of total RNA were used for each sample.

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VHL Status Influences Phenotype of Liver Cancers Arising from PTEN Loss

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2014-10-31 | E-GEOD-55874 | biostudies-arrayexpress

Homo sapiens

Project description:microRNA deregulation in Hepatocellular Carcinoma

| PRJNA140683 | ENA

Expression of Epithelial-to-Mesenchymal Transition (EMT)-associated genes in extratumoral microenvironment predicts poor clinical outcome in breast cancer patients

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2011-12-31 | E-GEOD-31589 | biostudies-arrayexpress

mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

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2011-02-17 | E-GEOD-20679 | biostudies-arrayexpress

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