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Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [scRNA-Seq]

ABSTRACT: Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [scRNA-Seq]

PROVIDER: PRJNA666791 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12755872.fastq.gz	Fastqsanger.gz
	SRR12755873.fastq.gz	Fastqsanger.gz
	SRR12755874.fastq.gz	Fastqsanger.gz
	SRR12755875.fastq.gz	Fastqsanger.gz
	SRR12755876.fastq.gz	Fastqsanger.gz

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Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration

Project description:Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration

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Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [RNA-Seq]

Project description:Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [RNA-Seq]

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Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [ATAC-Seq]

Project description:Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [ATAC-Seq]

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Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [scATAC-Seq]

Project description:Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [scATAC-Seq]

| PRJNA666800 | ENA

Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [scATAC-Seq]

Project description:Adult liver has enormous regenerative capacity as it can regenerate after losing two-thirds of its mass while sustaining essential metabolic functions. How the liver balances dual demands for increased proliferative activity with maintenance of organ function is unknown, but essential to prevent liver failure. Using partial hepatectomy (PHx) in mice to model liver regeneration, we integrated single-cell RNA and ATAC sequencing to map state transitions in ~ 13,000 hepatocytes at single-cell resolution as livers regenerated, and validated key findings with immunohistochemistry, to uncover how the organ regenerates hepatocytes while simultaneously fulfilling its vital tissue-specific functions. After PHx, hepatocytes rapidly and transiently diversified into multiple distinct populations with distinct functional bifurcation: some retained the chromatin landscapes and transcriptomes of hepatocytes in undamaged adult livers while others transitioned to acquire chromatin landscapes and transcriptomes of fetal hepatocytes. Injury-related signaling pathways known to be critical for regeneration were activated in transitioning hepatocytes and the most fetal-like hepatocytes exhibited chromatin landscapes that were enriched with transcription factors regulated by those pathways.

2020-10-02 | GSE158873 | GEO

Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [scRNA-Seq]

2020-10-02 | GSE158866 | GEO

Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [ATAC-Seq]

2020-10-02 | GSE158865 | GEO

Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration [RNA-Seq]

2020-10-02 | GSE158864 | GEO

Single-cell omics analysis reveals functional diversification of hepatocytes during liver regeneration

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-10-02 | GSE158874 | GEO

Single Cell triple omics sequencing reveals genetic and epigenetic heterogeneity in hepatocellular carcinoma

Project description:Single cell genome, DNA methylome, and transcriptome sequencing has been achieved separately. However, to analyze the regulation of RNA expression by genetic and epigenetic factors within an individual cell, it is necessary to analyze these omics simultaneously from the same single cell. Here we developed a single cell triple omics sequencing technique- scTrio-seq, to analyze the genome, DNA methylome, and transcriptome concurrently of a mammalian cell. 6 single human HepG2 cell line cells were sequenced using the newly developed scTrio-seq, other 2 HepG2 cells were sequenced using scRNA-seq and other 2 HepG2 cells were sequenced using scRRBS as technique control. 6 single mouse embryonic stem cells (mESCs) were sequenced using the newly developted scTrio-seq. Meanwhile, two scRNA-seq and two scRRBS were also completed using two mESCs separately. 26 single cells from hepatocellular carcinoma were sequenced using scTrio-seq to analyze the regulation relations between three omics of cancer cells.

2016-03-01 | E-GEOD-65364 | biostudies-arrayexpress

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