Project description:Analysis of skeletal muscle satellite cells with specific knockout (KO) of Carnitine Palmitoyltransferase 2 (Cpt2) gene in mouse (named Cpt2PKO). Cpt2 knockout disrupts fatty acid oxidation in satellite cells and causes energy insufficiency and alteration of protein acetylation, which eventually impedes their expansion and differentiation, leading to impairments in muscle regeneration.
Project description:Analysis of skeletal muscle satellite cells with specific knockout (KO) of Patatin-like phospholipase domain containing protein 2 (Pnpla2) gene in mouse (named Pnpla2PKO). Pnpla2 knockout disrupts lipid droplet catabolism in satellite cells and causes energy insufficiency and oxidative stress, accompanied by global lipid remodeling, which eventually impedes their expansion and fusion, leading to impairments in muscle regeneration.
Project description:Interventions: experimental group :PD-1 Knockout Engineered T Cells
Primary outcome(s): Number of participants with Adverse Events and/or Dose Limiting Toxicities as a Measure of Safety and tolerability of dose of PD-1 Knockout T cells using Common Terminology Criteria for Adverse Events (CTCAE v4.0) in patients
Study Design: historical control
Project description:Enhancing the proliferation and myogenic commitment of progenitor cells during fetal development enhances muscle growth and lean production in offspring. During the early development, a pool of skeletal stem cells (SSCs) proliferates and then diverge into either myoblast. Myoblast further fusion and develop into myotube. However, the landscape from muscle stem cells to myotubes in goats is not yet clear. This study aims to characterize the changes in the expression profile of skeletal muscle stem cells that differentiate into myoblasts, then migrate and fuse to form myotubes.
