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Phalaenopsis equestris cultivar:blue

ABSTRACT: Transcriptome atlas of Phalaenopsis equestris

PROVIDER: PRJNA667255 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13734100.fastq.gz	Fastqsanger.gz
	SRR13734101.fastq.gz	Fastqsanger.gz
	SRR13734102.fastq.gz	Fastqsanger.gz
	SRR13734103.fastq.gz	Fastqsanger.gz
	SRR13734104.fastq.gz	Fastqsanger.gz

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Project description:The F1 population of Phalaenopsis Intermedia, which established from the cross between P. equestris and P. aphrodite was applied to build a high-density genetic map based on SSRs and SNPs from GBS methodology, with the digestion of by Hinp1 I and Hae III. In addition, another GBS was performed with enzymes ApeK I and Hae II to increase the SNP number for the GWAS analysis. We identified 10 SNPs highly associated with floral aesthetic trait, and among them, 4 were associated with flower color related. Genes with function related to anthocyanin biosynthesis were identified as the candidate genes. In addition, the flowering time-related gene SOC1 may contribute to flower color regulation in our discovery.

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Transcriptome atlas of <i>Phalaenopsis equestris</i>.

Project description:The vast diversity of Orchidaceae together with sophisticated adaptations to pollinators and other unique features make this family an attractive model for evolutionary and functional studies. The sequenced genome of Phalaenopsis equestris facilitates Orchidaceae research. Here, we present an RNA-seq-based transcriptome map of P. equestris that covers 19 organs of the plant, including leaves, roots, floral organs and the shoot apical meristem. We demonstrated the high quality of the data and showed the similarity of the P. equestris transcriptome map with the gene expression atlases of other plants. The transcriptome map can be easily accessed through our database Transcriptome Variation Analysis (TraVA) for visualizing gene expression profiles. As an example of the application, we analyzed the expression of Phalaenopsis "orphan" genes-those that do not have recognizable similarity with the genes of other plants. We found that approximately half of these genes were not expressed; the ones that were expressed were predominantly expressed in reproductive structures.

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