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Next Generation Sequencing based mRNA sequencing of osteogenic differentiation of human mesenchymal stem cells from hair follicle outer root sheath (MSCORS)

ABSTRACT: Next Generation Sequencing based mRNA sequencing of osteogenic differentiation of human mesenchymal stem cells from hair follicle outer root sheath (MSCORS)

PROVIDER: PRJNA667751 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12782314_1.fastq.gz	Fastqsanger.gz
	SRR12782314_2.fastq.gz	Fastqsanger.gz
	SRR12782315_1.fastq.gz	Fastqsanger.gz
	SRR12782315_2.fastq.gz	Fastqsanger.gz
	SRR12782316_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing based mRNA sequencing of human mesenchymal stem cells from hair follicle outer root sheath

Project description:Next Generation Sequencing (NGS) based mRNA sequencing (RNA-seq) has provided high-throughput measurements to analyze the transcriptome of cells. We report the comprehensive transcriptome profiling of primarily isolated human mesenchymal stem cells from hair follicle outer root sheath (MSCORS) in series cultivation passages using NGS-based RNA-seq method. Human anagen hair follicles were non-invasively plucked from scalp of healthy donors, cultivated on a Transwell membrane, from which the MSCORS were isolated and subcultured onto normal culture flask. Attached and proliferating MSCORS were cultivated and characterized as mesenchymal stem cells (MSC) according to the minimum criteria for defining multipotent MSC from the International Society for Cellular Therapy (ISCT). mRNA profiles of MSCORS in p0, p1 and p2 were quality controlled, and generated by deep sequencing, in triplicate, using Illumina PE150. Paired-end raw data were pre-processed using a published protocol. HISAT2 (Hierarchical Indexing for Spliced Alignment of Transcripts) was used to align reference genome. HTSeq was used to calculate Reads Count, and gene abundence was determined by analyzing FPKM (fragments per kilobase of exon model per million reads mapped).

2020-10-07 | GSE159139 | GEO

Gene expression profiling of the intact dermal sheath cup of human hair follicle

Project description:The dermal sheath cup is the peribulbar component of the hair follicle dermal sheath, and has hair inductive potential similar to the dermal papilla. To characterize it in comparison with other mesenchymal follicle tissuesparts, we performed gene expression profiling of intact dermal sheath cups, which were separated from hair follicles by microdissection. Gene expression profiles of the dermal sheath cup, dermal papilla and upper dermal sheath were compared. We identified a dermal sheath cup signature composed of 32 upregulated genes, which included extracellular matrix components and BMP binding mollecules, while dermal papilla signature included a number of dermal papilla signature genes which had already reported. Analyses of upstream regulators showed that TGF- b1 is a putative regulator of these genes. These results suggest some of molecular mechanism that contributes to human dermal sheath cup properties, which could be useful for hair follicle bioengineering.

2020-02-22 | GSE95219 | GEO

Next Generation Sequencing based mRNA sequencing of human mesenchymal stem cells from hair follicle outer root sheath

Project description:Next Generation Sequencing based mRNA sequencing of human mesenchymal stem cells from hair follicle outer root sheath

| PRJNA667750 | ENA

Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression

Project description:Progenitor cell death and dermal papilla relocation during the regression phase of the hair growth cycle are essential for stem cell activation and follicle regeneration. This drastic follicle remodeling is coordinated by dermal sheath (DS) smooth muscle contraction, but how DS-generated forces are regulated is unknown. Here, we identify endothelin signaling - a potent vasoconstriction-regulating pathway - as the key activating mechanism of DS contraction. Pharmacological blocking or genetic ablation of both endothelin receptors, ETA and ETB, impedes DS contraction and halts follicle regression. Progenitors at the epithelial strand bottleneck are the main source of endothelin ligand ET-1, which is required for follicle regression. ET signaling in DS cells and downstream contraction is dynamically regulated by cytoplasmic Ca2+ levels via cell membrane and sarcoplasmic reticulum calcium channels. Together, these findings illuminate an epithelial-mesenchymal-interaction paradigm in which progenitors - before undergoing programmed cell death - control the contraction of the surrounding sheath smooth muscle to orchestrate homeostatic tissue regression and follicle reorganization.

2022-10-31 | GSE215133 | GEO

Next Generation Sequencing based mRNA sequencing of osteogenic differentiation of human mesenchymal stem cells from hair follicle outer root sheath (MSCORS)

Project description:Next Generation Sequencing (NGS)-based mRNA sequencing (RNA-seq) has provided high-throughput measurements OF MRNA COPIES? to analyze the transcriptome of cells. We are reporting comprehensive transcriptome profiling of primarily isolated human adipose-derived mesenchymal stem cells (ADMSC) throughout consecutive culture passages using NGS-based RNA-seq method. Human adipose tissue was obtained from operative remains and and digested in collagenase X. ADMSC were isolated, cultivated and characterized as mesenchymal stem cells (MSC) according to the minimum criteria for defining multipotent MSC from the International Society for Cellular Therapy (ISCT).The cells were subjected to osteogenic differentiation for 1, 2 and 3 weeks. mRNA profiles of differentiated MSCORS afer 1, 2, and 3 weeks were quality controlled, and generated by deep sequencing, in triplicate, using Illumina PE150 kits. Paired-end raw data were pre-processed using a published protocol. HISAT2 (Hierarchical Indexing for Spliced Alignment of Transcripts) was used to align reference genome. HTSeq was used to calculate Reads Count, and gene abundence was determined by analyzing FPKM (fragments per kilobase of exon model per million reads mapped).

2020-10-07 | GSE159140 | GEO

Gene expression profiling of adult hair follicle dermal stem cells and progeny, dermal sheath and dermal papilla cells

Project description:Here we have developed a novel FACS strategy to prospectively isolate hair follicle dermal stem cells, dermal sheath and dermal papilla cells from adult skin initiating synchronous hair follicle regeneration and identified Hic1 as a marker of hfDSCs and Rspondins as stimulators of hfDSCs and epithelial cells, and subsequently hair follicle regeneration

2020-02-03 | GSE109256 | GEO

Replication Stress in Hair-Follicle-Stem-Cell Leads to IFI16-STING dependent inflammation in Hidradenitis Suppurativa

Project description:Hidradenitis suppurativa (HS) is a chronic, relapsing, inflammatory skin disease. We characterized hair follicle stem cells isolated from HS and healthy (HD) patients and more precisely the Outer Root Sheath Cells (ORS). We show that transcriptomic analyses of hair follicles from HS (n=6) and HD (n=5) revealed an interferon signature and an alteration of cell cycle pathways

2020-07-08 | GSE147586 | GEO

Less invasive diagnostics for cancer using hair follicle

Project description:Primary outcome(s): Comparison of the gene expression profile in hair follicle between cancer patients and healthy volunteer

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Transcription profiling of different types of cells in murine hair follicle to study cell type-specific gene expression.

Project description:Hair follicle matrix, outer root sheath, dermal papilla cells and melanocytes and a dermal fraction enriched in fibroblasts were FACS isolated from 4d backskins. Targets from two biological replicates of each were generated and the expression profiles were determined using Affymetrix Mouse Genechip 430A arrays. Comparisons between the sample groups allow the identification of cell-type specific genes. Experiment Overall Design: Mx, ORS, DF, DP and Mc were FACSorted from Lef1-RFP/K14-H2BGFP mice from 4 day old backskins

2007-09-07 | E-GEOD-3142 | biostudies-arrayexpress

Dermal sheath contraction powers stem cell niche relocation during hair cycle regression

Project description:Tissue homeostasis requires the balance of growth by cell production and regression through cellular loss. In the hair cycle during follicle regression, the niche traverses the skin through an unknown mechanism to reach the stem cells and prime regeneration. Here, by cell specific ablation and intravital imaging in live mice, we identify the follicle-lining dermal sheath as the key driver of tissue regression and niche relocation via smooth muscle contractile machinery that generates centripetal constriction force. We reveal the calcium/calmodulin/myosin light chain kinase pathway as the mechanism of sheath contraction that when blocked inhibits follicle contraction and impedes regression and niche relocation. Thus, our study identifies the dermal sheath as a smooth muscle that drives regression for reuniting niche and stem cells to regenerate tissue structure during homeostasis.

2019-12-31 | GSE136996 | GEO

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