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Effects of temperature change within the readily tolerated range on transcription and DNA accessibility in the Drosophila S2R+ cell line

ABSTRACT: Effects of temperature change within the readily tolerated range on transcription and DNA accessibility in the Drosophila S2R+ cell line

PROVIDER: PRJNA667915 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12794422_1.fastq.gz	Fastqsanger.gz
	SRR12794422_2.fastq.gz	Fastqsanger.gz
	SRR12794423_1.fastq.gz	Fastqsanger.gz
	SRR12794423_2.fastq.gz	Fastqsanger.gz
	SRR12794424_1.fastq.gz	Fastqsanger.gz

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Effect of histone H2Av depletion on temperature dependence of the Drosophila S2R+ cell transcriptome

Project description:The histone H2A variant H2A.Z has a crucial role in the regulation of temperature-dependent gene expression in the plant Arabidopsis thaliana. To evaluate whether the Drosophila homolog histone H2Av has a similar role in the control of the transcriptional response to temperature change, we depleted this histone variant by RNA interference in S2R+ cells and analyzed the effect on the transcriptome after temperature shifts to different temperatures (14, 25 and 30°C). For control, we performed analogous experiments using treatment with lacZ dsRNA instead of His2Av dsRNA.

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Effects of temperature change within the readily tolerated range on transcription and DNA accessibility in the Drosophila S2R+ cell line

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-09-13 | GSE159174 | GEO

Temporal dynamics of transcriptome change in Drosophila S2R+ cells after a temperature shift from 25 to 14°C

Project description:Temporal dynamics of transcriptome change in Drosophila S2R+ cells after a temperature shift from 25 to 14°C

| PRJNA668005 | ENA

ChIP-seq analysis of M1BP in Drosophila S2R+ cells

Project description:The genomic distribution of a novel transcription factor called M1BP was determined in Drosophila S2R+ cells

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Time series RNA-seq analyses of Drosophila S2R+ cells after insulin stimulation

Project description:Purpose: identifying genes responding to insulin stimulation in S2R+ cells through whole transcriptome RNA-seq analyses Methods: Total RNA was extracted from S2R+ cells using TRIzol® reagent (Invitrogen). After assessing RNA quality with an Agilent Bioanalyzer, libraries were constructed with Illumina TruSeq mRNA Library Prep Kit , libraries were sequenced using an Illumina HiSeq 4000 at the Columbia Genome Center (http://systemsbiology.columbia.edu/genome-center). Results: Using an time series data analysis workflow incorporating polynormials , we identified 1254 temproally differentially expressed genes responding to insulin stimulation in the S2R+ cells.

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