Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of ambient temperature on DNA accessibility in nuclear chromatin of Drosophila S2R+ cells [ATAC-seq]

ABSTRACT: Effects of ambient temperature on DNA accessibility in nuclear chromatin of Drosophila S2R+ cells [ATAC-seq]

PROVIDER: PRJNA668073 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12794422_1.fastq.gz	Fastqsanger.gz
	SRR12794422_2.fastq.gz	Fastqsanger.gz
	SRR12794423_1.fastq.gz	Fastqsanger.gz
	SRR12794423_2.fastq.gz	Fastqsanger.gz
	SRR12794424_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Effects of ambient temperature on DNA accessibility in nuclear chromatin of Drosophila S2R+ cells [ATAC-seq]

Project description:Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to ambient temperature change is predicted to involve complex regulation, including the transcriptional level. To study effects of changes in ambient temperature on chromatin accessibility, we performed ATAC-Seq with S2R+ cells, a line derived from embryos of the ectothermic organism Drosophila melanogaster. Aliquots of S2R+ cells were exposed to different temperatures (14, 25 and 29°C) within the readily tolerated range before analysis with ATAC-Seq.

2021-09-13 | GSE159221 | GEO

Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells

Project description:Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to ambient temperature change is predicted to involve complex regulation. To assess the effects of temperature change within the readily tolerated temperature range on the transcriptome, we have performed expression profiling with S2R+ cells, which are derived from the ectothermic organism Drosophila melanogaster.

2021-09-13 | GSE159170 | GEO

Effects of ambient temperature on transcript levels and alternative polyadenylation in Drosophila melanogaster [RNA-seq]

Project description:Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to ambient temperature change is predicted to involve complex regulation. To assess the effects of temperature change within the readily tolerated range on the transcriptome, we have performed analyses with the ectothermic organism Drosophila melanogaster. Both adult male flies and S2R+ cells were analyzed. 3' RNA-Seq was applied to study effects of ambient temperature change on transcript levels and on polyadenylation site selection.

2021-09-13 | GSE159222 | GEO

Temporal dynamics of transcriptome change in Drosophila S2R+ cells after a temperature shift from 25 to 14°C

Project description:Cells in ectothermic organisms often maintain homeostatic function over a considerable range of ambient temperatures. However, as temperature has pronounced effects on all biological processes, but not necessarily in a uniform manner on each of the myriad of distinct processes, cellular acclimation to distinct temperatures is predicted to involve complex regulation. To assess the effects of a temperature downshift from 25 to 14°C, i.e. from the optimal temperature to the lower limit of the readily tolerated range, on the transcriptome in a time-resolved manner, we have performed expression profiling with S2R+ cells, which are derived from the ectothermic organism Drosophila melanogaster.

2021-09-13 | GSE159172 | GEO

Effect of histone H2Av depletion on temperature dependence of the Drosophila S2R+ cell transcriptome

Project description:The histone H2A variant H2A.Z has a crucial role in the regulation of temperature-dependent gene expression in the plant Arabidopsis thaliana. To evaluate whether the Drosophila homolog histone H2Av has a similar role in the control of the transcriptional response to temperature change, we depleted this histone variant by RNA interference in S2R+ cells and analyzed the effect on the transcriptome after temperature shifts to different temperatures (14, 25 and 30°C). For control, we performed analogous experiments using treatment with lacZ dsRNA instead of His2Av dsRNA.

2021-09-13 | GSE159171 | GEO

Long-Term Storage of Human Cells at Ambient Temperature

Project description:This series represents the complete series of the human 293h media depleted storage on agarose / rehydration condition course analysis. Samples include Control, monolayer; Control, monolayer/full recovery, antibiotics; Spheroid, no storage; two week storage/0hr recovery; two week storage/full recovery; four week storage/0hr recovery; six week storage/0hr recovery. Keywords = 293h cells Keywords = desiccation Keywords = rehydration Keywords = spheroid Keywords = stabilization Keywords = ambient temperature Keywords: other

2005-08-01 | GSE1364 | GEO

Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells

Project description:Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells

| PRJNA668006 | ENA

Two week Storage and Rehydration of HEK 293 Cells at Ambient Temperature

Project description:This series represents the rehydration series of the human 293h media depleted storage on agarose / rehydration condition course analysis. Samples include Control Monolayer, 0 hr desiccation, 0 hr rehydration, 6 hr rehydration, 24 hr rehydration, and 72 hr rehydration. Keywords = 293h cells Keywords = desiccation Keywords = rehydration Keywords = spheroid Keywords = stabilization Keywords = ambient temperature Keywords: other

2005-08-01 | GSE1455 | GEO

Study of the functional implication of altered nuclear morphology on global gene expression profile in Drosophila S2R+ cells

Project description:A probe of the coupling of gene expression and nuclear structure in Drosophila S2R+ cells was undertaken by a study of altered expression profile following a 6 day RNAi mediated genetic perturbation of Klaroid (CG18584), Lamin (CG6944) and Msp-300 (CG33715).

2012-10-01 | GSE32525 | GEO

Study of the functional implication of altered nuclear morphology on global gene expression profile in Drosophila S2R+ cells

2012-10-01 | E-GEOD-32525 | biostudies-arrayexpress