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Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine

ABSTRACT: Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine

PROVIDER: PRJNA668385 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12802800_1.fastq.gz	Fastqsanger.gz
	SRR12802800_2.fastq.gz	Fastqsanger.gz
	SRR12802801_1.fastq.gz	Fastqsanger.gz
	SRR12802801_2.fastq.gz	Fastqsanger.gz
	SRR12802802_1.fastq.gz	Fastqsanger.gz

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Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [Ribo-seq]

Project description:Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [Ribo-seq]

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Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [RNA-seq]

Project description:Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [RNA-seq]

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Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine

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2021-05-05 | GSE159338 | GEO

Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [Ribo-seq]

Project description:We analyzed total RNA and ribosome footprints collected from a K. phaffii strain secreting the VP8-P[6] serotype of the rotavirus antigen and compared against a strain secreting the VP8-P[4] serotype for reference.

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Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [RNA-seq]

Project description:We analyzed the transcriptomes of K. phaffii strains secreting the VP8-P[6] serotype of the rotavirus antigen, and compared against null strains as well as strains secreting the VP8-P[4] or VP8-P[8] serotype in order to diagnose bottlenecks limiting robust secretion of P[6].

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Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus.

Project description:BackgroundVaccines comprising recombinant subunit proteins are well-suited to low-cost and high-volume production for global use. The design of manufacturing processes to produce subunit vaccines depends, however, on the inherent biophysical traits presented by an individual antigen of interest. New candidate antigens typically require developing custom processes for each one and may require unique steps to ensure sufficient yields without product-related variants.ResultsWe describe a holistic approach for the molecular design of recombinant protein antigens-considering both their manufacturability and antigenicity-informed by bioinformatic analyses such as RNA-seq, ribosome profiling, and sequence-based prediction tools. We demonstrate this approach by engineering the product sequences of a trivalent non-replicating rotavirus vaccine (NRRV) candidate to improve titers and mitigate product variants caused by N-terminal truncation, hypermannosylation, and aggregation. The three engineered NRRV antigens retained their original antigenicity and immunogenicity, while their improved manufacturability enabled concomitant production and purification of all three serotypes in a single, end-to-end perfusion-based process using the biotechnical yeast Komagataella phaffii.ConclusionsThis study demonstrates that molecular engineering of subunit antigens using advanced genomic methods can facilitate their manufacturing in continuous production. Such capabilities have potential to lower the cost and volumetric requirements in manufacturing vaccines based on recombinant protein subunits.

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