Project description:Genome wide DNA methylation profiling in cutaneous squamous cell carcinoma. The Infinium MethylationEPIC BeadChips 850K has been used to interrogate DNA methylation changes. The cohort included 23 patients in total, with precursors of squamous cell carcinoma (actinic keratosis group) and with cSCC at different stages.
Project description:To determine the differentially expressed miRNAs in human cutaneous squamous cell carcinoma biopsies versus normal skin samples using microarray analysis.
Project description:In this study, we interrogated the changes in the fibronectin-induced adhesome in cancer cells using patient-derived malignant keratinocytes from cutaneous squamous cell carcinoma (cSCC). We found that the human enabled homologue, Mena (encoded by ENAH; also known as hMena), an actin regulatory protein of the Ena/VASP family, is upregulated in the adhesome of metastatic cSCC cells compared to equivalent cells from the primary tumour. Using a network analysis approach, we found that Mena clusters within an actin-binding/regulatory module that is upregulated in metastatic keratinocytes. Our proteomic analysis connected Mena to the LINC complex component nesprin-2, and we uncovered a novel role for Mena at the perinuclear region of metastatic keratinocytes. We found that Mena locates adjacent to, and interacts with the C-terminal spectrin repeats of, nesprin-2 at the nuclear membrane. Mena loss results in the reduction of nesprin-2 interactions with actin and lamin A/C, affecting nuclear morphology. Moreover, Mena loss results in the reduction of emerin tyrosine phosphorylation and affects the regulation of PTX3 (which encodes pentraxin-3, an immunomodulatory component of the complement system) via the recruitment of its putative enhancer region to the nuclear lamina
Project description:The expression of lncRNAs in cutaneous squamous cell carcinoma (cSCC) were determined with a commercially available microarray (Arraystar Inc.,Rockville, Maryland USA). The Arraystar human lncRNA Microarray V3.0 containing 30,586 LncRNAs and 26,109 coding transcripts (mRNAs)