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A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae

ABSTRACT: A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae

PROVIDER: PRJNA669988 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12828452_1.fastq.gz	Fastqsanger.gz
	SRR12828452_2.fastq.gz	Fastqsanger.gz
	SRR12828453_1.fastq.gz	Fastqsanger.gz
	SRR12828453_2.fastq.gz	Fastqsanger.gz
	SRR12828454_1.fastq.gz	Fastqsanger.gz

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Project description:Mitochondrial ribosomes (mitoribosomes) have undergone substantial structural remodelling throughout evolution leading to distinct structural properties compared to their prokaryotic ancestors. Compared to their prokaryotic counterparts, mitoribosomes show a substantial loss of ribosomal RNA (rRNA), whilst acquiring unique mitochondrial proteins located on the periphery of the mitoribosome. This extended protein content suggests mitochondrial-specific functions of these proteins involved in mitochondrial mRNA selective-translation activation, ribosome assembly, translation regulation rather than exclusively structural scaffolding. We investigated the functional properties of the 14 unique (mitochondria-specific or supernumerary) mammalian mitoribosomal proteins (snMRP) in the small mitochondrial subunit (mtSSU) by CRISPR-mediated knockout and describe their specific effect on mitoribosome integrity and function. Unexpectedly, we show that each snMRP knockout leads to a unique pattern of mitoribosome instability with variable effects on mitochondrial protein synthesis as essential structural components and all – with the exception of mS37 (MRPS37, CHCHD1) – to almost complete loss of protein synthesis. Concomitantly, through steady-state proteomic analysis we confirm a modular assembly of the mtSSU. Surprisingly, mS37 was found to have impaired stability in all our tested mtSSU and large mitochondrial subunit (mtLSU) snMRPs knockouts as well as in patient-derived cell lines, suggesting an extended role of the protein in mtSSU pre-translation initiation steps. As mitochondrial intermembrane (IMS) targeted protein – a eukaryotic-confined localisation mechanism – with additional unique MIA40 (CHCHD4)-oxidisable motifs involved in the disulfide relay system, mS37 could have a specific role in mitochondrial translation regulation. Indeed, we find oxidation of the cysteines in the CX9C motif of mS37 to be essential for its stability, confirming mS37 regulating mitochondrial translation regulation.

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A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae

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