Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Molecular analysis of the subaortic hematopoietic stem cell niche [E10.5_2]

ABSTRACT: Molecular analysis of the subaortic hematopoietic stem cell niche [E10.5_2]

PROVIDER: PRJNA670010 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Molecular analysis of the subaortic hematopoietic stem cell niche

Project description:Molecular analysis of the subaortic hematopoietic stem cell niche

| PRJNA669831 | ENA

Molecular analysis of the subaortic hematopoietic stem cell niche [E11.5]

Project description:Molecular analysis of the subaortic hematopoietic stem cell niche [E11.5]

| PRJNA670009 | ENA

Molecular analysis of the subaortic hematopoietic stem cell niche [E12.5]

Project description:Molecular analysis of the subaortic hematopoietic stem cell niche [E12.5]

| PRJNA670008 | ENA

Molecular analysis of the subaortic hematopoietic stem cell niche [E10.5_1]

Project description:Molecular analysis of the subaortic hematopoietic stem cell niche [E10.5_1]

| PRJNA670012 | ENA

A neuro-mesenchymal cell subpopulation characterises the subaortic hematopoietic stem cell niche

Project description:A neuro-mesenchymal cell subpopulation characterises the subaortic hematopoietic stem cell niche

| PRJNA999307 | ENA

A neuro-mesenchymal cell subpopulation characterises the subaortic hematopoietic stem cell niche

Project description:The first hematopoietic stem and progenitor cells (HSPCs) emerge in the embryonic dorsal aorta of midgestation mouse embryo, but the precise nature of the supportive microenvironment remains largely unexplored. To address this question, we designed a laser microdissection-based approach to isolate the dorsal and ventral tissues of the aorta at three distinct developmental stages harboring HSPCs. Bulk and single cell transcriptomics combined with computational analyses disclosed a large mesenchymal cell population in the E11.5 subaortic tissue including a subset expressing interconnected genes implicated in adhesive and neuronal functions. In vivo lineage tracing showed that this subset does not derive from neural crest cells. Histological and functional studies validated our strategy and revealed that Decorin, and Tenascin C, two components of the extracellular matrix (ECM), are essential for HSPC development. Together, our study identifies a yet unrecognized subaortic mesenchymal cell population and underscores the critical role of the ECM on embryonic HSPCs.

2024-03-11 | GSE239434 | GEO

Molecular analysis of the subaortic hematopoietic stem cell niche [E12.5]

Project description:Hematopoietic stem cells (HSCs) are formed in the Aorta-Gonad-Mesobephros (AGM) region of mouse midgestation embryos. This process is tightly regulated in time and space by surrounding cells inculding mesenchymal cells located underneath the dorsal aorta. We combined laser microdissection and microarrays to isolate the dorsal and ventral aortic tissues at three developmental stages and to explore their gene expression profiling.

2024-03-11 | GSE159591 | GEO

Molecular analysis of the subaortic hematopoietic stem cell niche [E11.5]

2024-03-11 | GSE159590 | GEO

Molecular analysis of the subaortic hematopoietic stem cell niche [E10.5_2]

2024-03-11 | GSE159589 | GEO

Molecular analysis of the subaortic hematopoietic stem cell niche [E10.5_1]

2024-03-11 | GSE159588 | GEO