Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A comprehensive library of human transcription factors for cell fate engineering

ABSTRACT: A comprehensive library of human transcription factors for cell fate engineering

PROVIDER: PRJNA670483 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12270000.fastq.gz	Fastqsanger.gz
	SRR12270001.fastq.gz	Fastqsanger.gz
	SRR12270002.fastq.gz	Fastqsanger.gz
	SRR12270003.fastq.gz	Fastqsanger.gz
	SRR12270004.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 1727

Similar Datasets

A comprehensive library of human transcription factors for cell fate engineering (scRNA-Seq)

Project description:A comprehensive library of human transcription factors for cell fate engineering (scRNA-Seq)

| PRJNA670567 | ENA

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen CRTD5)

Project description:A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen CRTD5)

| PRJNA670481 | ENA

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen ATCC)

Project description:A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen ATCC)

| PRJNA670479 | ENA

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen PGP1)

Project description:A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen PGP1)

| PRJNA670566 | ENA

A comprehensive library of human transcription factors for cell fate engineering (Bulk RNA-Seq)

Project description:A comprehensive library of human transcription factors for cell fate engineering (Bulk RNA-Seq)

| PRJNA670478 | ENA

A comprehensive library of human transcription factors for cell fate engineering (scRNA-Seq)

Project description:Human pluripotent stem cell (hPSC)-derived cells offer an unprecedented opportunity to model diverse human tissue functions. However, the field lacks a generalized approach to rapidly and efficiently generate individual cell types of interest and to incorporate them into complex but defined tissues. To systematically explore the transcription factor (TF)-mediated hPSC programming landscape, we constructed the Human TFome: a comprehensive human TF library containing 1,564 TF (1,732 splice-isoforms). We discovered 241 previously unreported factors that individually converted hPSCs into diverse lineages with up to 99% efficiency in four days without alteration of external factors. Among these, we programmed neurons, fibroblasts, oligodendrocytes and endothelial cells that have molecular, transcriptomic and functional similarity to primary cells. Splice-isoform-specificity doubled endothelial conversion efficiency into in vivo-functional cells. Our cell-autonomous approach enabled parallel and orthogonal hPSCs programming into multiple cell types simultaneously without altering any microenvironmental cues. We generated in vivo-engraftable induced oligodendrocytes that expedited myelination within synthetically accelerated cerebral organoids.

2020-10-22 | GSE159784 | GEO