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Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [scRNA-seq]

ABSTRACT: Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration [scRNA-seq]

PROVIDER: PRJNA670657 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12875650_1.fastq.gz	Fastqsanger.gz
	SRR12875650_2.fastq.gz	Fastqsanger.gz

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Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration

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Project description:Tissue regeneration is a process that recapitulates and restores organ structure and function. Although previous studies have demonstrated wound-induced hair neogenesis (WIHN) in laboratory mice (Mus), the regeneration is limited to the center of the wound unlike those observed in African spiny (Acomys) mice. Tissue mechanics have been implicated as an integral part of tissue morphogenesis. Here, we use the WIHN model to investigate the mechanical and molecular responses of laboratory and African spiny mice, and report these models demonstrate opposing trends in spatiotemporal morphogenetic field formation with association to wound stiffness landscapes. Transcriptome analysis and K14-Cre-Twist1 transgenic mice show the Twist1 pathway acts as a mediator for both epidermal-dermal interactions and a competence factor for periodic patterning, differing from those used in development. We propose a Turing model based on tissue stiffness that supports a two-scale tissue mechanics process: (1) establishing a morphogenetic field within the wound bed (mm scale) and (2) symmetry breaking of the epidermis and forming periodically arranged hair primordia within the morphogenetic field (μm scale). Thus, we delineate distinct chemo-mechanical events in building a Turing morphogenesis-competent field during WIHN of laboratory and African spiny mice and identify its evo-devo advantages with perspectives for regenerative medicine.

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