Project description:Renibacterium salmoninarum is a Gram-positive, intracellular bacterial pathogen that causes Bacterial Kidney Disease (BKD) in Atlantic salmon (Salmo salar). To identify R. salmoninarum bacterin-responsive genes in Atlantic salmon, fish (n=6) were intraperitoneally injected with a high (5×107 cells/kg; High-Rs) dose of formalin-killed R. salmoninarum or phosphate-buffered saline (PBS control). Head kidney samples were collected at 24 h post-injection. Using 44K microarray analysis, transcriptome analysis was performed to identify differentially expressed probes in response to formalin-killed R. salmoninarum injection.
Project description:Renibacterium salmoninarum is a Gram-positive, intracellular bacterial pathogen that causes Bacterial Kidney Disease (BKD) in Atlantic salmon (Salmo salar). To identify Atlantic salmon genes responsive to R. salmoninarum pathogen, fish were intraperitoneally injected with R. salmoninarum (BKD) or sterile KDM-2 medium (control). Head kidney samples were collected at 13 days post-infection. Using 44K microarray analysis, transcriptome analysis was performed to identify differentially expressed probes in response to different levels of R. salmoninarum infection.
