Project description:Long-term hematopoietic stem cells are rare, highly quiescent stem cells of the hematopoietic system with life-long self-renewal potential and the ability to transplant and reconstitute the entire hematopoietic system of conditioned recipients. Most of our understanding of these rare cells has relied on cell surface identification, epigenetic and transcriptomic analyses. Our knowledge of protein synthesis, folding, modification and degradation – broadly termed protein homeostasis or “proteostasis” – in these cells is still in its infancy. Here we report the requirement of the small phospho-binding adaptor proteins, the cyclin dependent kinase subunits (Cks1 and Cks2), for maintaining ordered hematopoiesis and long-term hematopoietic stem cell reconstitution. Cks1 and Cks2 are critical regulators of a myriad of key intracellular signalling pathways that govern hematopoietic stem cell biology and together they balance protein homeostasis and restrain reactive oxygen species to ensure healthy hematopoietic stem cell function.

2023-03-11 | PXD035984 | Pride

Epigenetic analyses of hematopoietic stem cells

Project description:We analyzed genome-wide chromatin accessibility in hematopoietic stem cells (HSCs) using ATAC-seq in mice after sham operation and transverse aortic constriction (TAC).

2024-02-13 | GSE160067 | GEO

Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells

Project description:Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells

| PRJNA682581 | ENA

Casirati et al. Epitope Editing of Hematopoietic Stem Cells

Project description:Data for the manuscript Casirati et al. "Epitope Editing of Hematopoietic Stem Cells Enables Adoptive Immunotherapies for Acute Myeloid Leukemia"

2023-06-26 | MSV000092272 | MassIVE

Analyses of barcode repertoire in hematopoietic cells

Project description:Analyses of barcode repertoire in hematopoietic cells

| PRJNA671729 | ENA

Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality

Project description:There are no described quality assurance mechanisms for newly formed stem cells. We observed intimate interactions between macrophages and blood stem cells in zebrafish embryos. Stressed stem cells were marked by surface Calreticulin, which stimulates macrophage interaction as an eat me signal. Macrophage-stem cell interactions either lead to removal of cytoplasmic material and stem cell proliferation or resulted in complete stem cell engulfment. Calreticulin knock down or embryonic macrophage depletion reduced the number of stem cell clones into adulthood. Our work supports a model in which embryonic macrophages determine hematopoietic clonality by monitoring stem cell quality.

2022-02-04 | MSV000088780 | MassIVE

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