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High-throughput RNA Sequencing of THP-1 macrophages treated with or without native LDL for 24h.

ABSTRACT: High-throughput RNA Sequencing of THP-1 macrophages treated with or without native LDL for 24h.

PROVIDER: PRJNA673803 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR12964433_1.fastq.gz	Fastqsanger.gz
	SRR12964433_2.fastq.gz	Fastqsanger.gz
	SRR12964434_1.fastq.gz	Fastqsanger.gz
	SRR12964434_2.fastq.gz	Fastqsanger.gz
	SRR12964435_1.fastq.gz	Fastqsanger.gz

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Project description:We previously found that a native lipoprotein mix with a high VLDL+LDL/HDL ratio causes a global de novoDNA methylation in THP-1 macrophages. In the present experiment we assessed the consequences of global lipoprotein-induced de novo DNA methylation on global gene expression in the same cells. Moreover, we sought to use gene expression array data to measure RNA expression levels for candidate factors mediating the epigenetic effects of lipoproteins. Experiment Overall Design: Human native VLDL, LDL and HDL lipoproteins were isolated from buffy coats, fractionated by ultracentrifugation, stored at -80deg., desalted to PBS before usage and kept at 4deg. for a maximum of 7d. THP-1 monocytes were differentiated to macrophages, stimulated for 24h with a mix of 68.8mg/ml VLDL, 32.1mg/ml LDL, 91.1mg/ml HDL or unstimulated (control), in serum-free medium with 2% BSA. Three independently isolated lipoprotein samples were used in triplicate.

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