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Transcriptomics of THP-1 macrophages treated with or without IL-26 for 24h

ABSTRACT: Transcriptomics of THP-1 macrophages treated with or without IL-26 for 24h

PROVIDER: PRJNA791656 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17311829_1.fastq.gz	Fastqsanger.gz
	SRR17311829_2.fastq.gz	Fastqsanger.gz
	SRR17311830_1.fastq.gz	Fastqsanger.gz
	SRR17311830_2.fastq.gz	Fastqsanger.gz
	SRR17311831_1.fastq.gz	Fastqsanger.gz

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Transcriptomics of THP-1 macrophages treated with or without IL-26 for 24h

Project description:Purpose: The aim of this study was to obtain a comprehensive transcriptomic analysis of IL-26-exposed macrophages. Methods: THP-1 derived macrophage were generated by incubating THP-1 cells with PMA (50 ng/ml) for 24h.Then THP-1 macrophages were treated with IL-26 for 24h, which were collected and applied to RNA-sequencing. The constructed sequencing library was sequenced using an Illumina Novaseq 6000 system. The expression of transcripts was calculated as fragments per kilobase of exon model per million mapped reads (FPKM). Results: In total, we identified 1303 differentially expressed protein-coding genes between untreated and IL-26-treated macrophages, including 667 up-regulated and 636 down-regulated genes.

2022-04-27 | GSE192493 | GEO

RNA-seq of THP-1 cells stimulated with CpG-c and Hydroxychloroquine intervention

Project description:Purpose: To investigate the inhibition of inflammatoty cytokines of Hydroxychloroquine to phagocytic THP-1 cells. Methods: we established a cell model of hemophagocytosis by simulating THP-1 cells with CpG-c for 24h in vitro to produce macrophages, and then coculture the cells with human RBCs. RNA sequencing analysis of THP-1 cells were performed to find Gene changed with CpG-c stimulation and Hydroxychloroquine intervention. Results:CpG-c sitimulation significantly altered gene expression of THP-1 cells. Cytokine genes were upregulated, especially a significant increase in IL-6 level from scratch. HCQ significantly inhibited the expression of inflammatory cytokines and decreased IL-6 gene level to baseline. Conclusions:Hydroxychloroquine can inhibite cytokine genes, especially IL-6 Gene level of THP-1 cells caused by CpG-c sitimulation.

2022-07-01 | GSE198290 | GEO

RNA Sequencing of human THP-1 derived macrophages transcriptomes

Project description:THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA) at 25μg/ml for 48h.Then remove PMA and rest for 24h to obtain M0 macropahges. Further applied LPS and IFNγ or IL-4 and IL-13 for 24h to obtain M1, M2 macrophages, respectively. During polarization, cells were growth within control medium(LN) or medium with extra lipid mixture(LH).M0,M1,M2 cells were collected and applied to RNA-sequencing. We found that differential activation of macrophage had distinct metabolic signature.Extra lipids supplement did not significantly alter transcriptomes of macrophages.

2021-06-28 | GSE159112 | GEO

RNA Sequencing of PGE2 or MEHP treated human THP-1 derived macrophages transcriptomes

Project description:THP-1 cells were treated with phorbol 12-myristate 13-acetate (PMA) at 25μg/ml for 48h.Then remove PMA and rest for 24h to obtain M0 macropahges. Further applied LPS and IFNγ or IL-4 and IL-13 for 24h to obtain M1, M2 macrophages, respectively. During polarization, cells were treated with prostaglandin E2 or MEHP.Then M1,M2 cells were collected and applied to RNA-sequencing. We found that PGE2 and MEHP significantly impacted metabolic signature of macrophages.

2021-06-28 | GSE159120 | GEO

Neutrophils drive skin autoinflammation by releasing IL-26

Project description:Neutrophils drive skin autoinflammation by releasing IL-26

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High-throughput RNA Sequencing of THP-1 macrophages treated with or without native LDL for 24h.

Project description:High-throughput RNA Sequencing of THP-1 macrophages treated with or without native LDL for 24h.

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Effect of hyper-and hypoactivation of Notch signaling in IL-4-stimulate THP-1

Project description:MSCV-GFP-IRES (IRES), MSCV-GFP-Myc-NIC1 (NIC1) and MSCV-GFP-DNMAML (DNMAML) were retrovirally transduced in to THP-1 cell (pCL-Ampho was used as packaging plasmid). GFP positive cells were sorted for selective desired cell. Then IRES, NIC1 and DNMAML overexpressing THP-1 were pretreated with PMA (5ng/ml) for 48 h before stimulation with IL-4 (20 ng/ml) for 3 h.

2019-05-01 | GSE115914 | GEO

Interleukin-26 potentiates type 2 skin inflammation in presence of interleukin-1β

Project description:Atopic dermatitis (AD) is a debilitating inflammatory skin disorder. Biologics targeting the IL-4/IL-13 axis are effective in AD, but there is still a large proportion of patients that do not respond to IL-4R blockade. Further exploration of potentially pathogenic T cell-derived cytokines in AD may lead to new effective treatments. This study aimed to investigate the downstream effects of IL-26 on skin in the context of type 2 skin inflammation. We found that IL-26 alone exhibited limited inflammatory activity in skin. However, in presence of IL-1β, IL-26 potentiated the secretion of TSLP, CXCL1 and CCL20 from human epidermis through JAK/STAT signaling. Moreover, in an in vivo AD-like skin inflammation model, IL-26 exacerbated skin pathology and locally increased type 2 cytokines, most notably of Il13 in skin T helper cells. Neutralization of IL-1β abrogated IL-26-mediated effects, indicating that the presence of IL-1β is required for full IL-26 downstream action in vivo. These findings suggest that the presence of IL-1β enables IL-26 to be a key amplifier of inflammation in the skin. As such, IL-26 may contribute to the development and pathogenesis of inflammatory skin disorders such as AD.

2024-01-10 | GSE228860 | GEO

Interaction of IL-26 and its novel receptor EphA3 regulates EGFR-TKI resistance in triple negative breast cancer cells

Project description:We investigated how IL-26 stimulation affected gene expression in E0771 cells by DNA microarray analysis.

2021-04-01 | GSE147804 | GEO

Expression data from Il-27 stimulated normal human epidermal keratinocytes (NHEK) and THP-1 cells

Project description:IL-27 plays a pivotal role in regulating complex immune responses. To evaluate the role of IL-27 in modulation in the expression of cellular and innate immunity-asscoiated genes in human epidermal keratinocytes (primary cells) and the monocytic THP-1 cell line (pre-treated with PMA) were stimulated with IL-27 and genome-wide expression of these cells were investigated utilizing microarray assay.

2020-01-08 | GSE143228 | GEO

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