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RNA sequencing of single blastocyst-stage embryos (E3.5) from zygotes which were injected with sperm sRNAs from normal or depressive male mice.

ABSTRACT: RNA sequencing of single blastocyst-stage embryos (E3.5) from zygotes which were injected with sperm sRNAs from normal or depressive male mice.

PROVIDER: PRJNA674788 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12992006_1.fastq.gz	Fastqsanger.gz
	SRR12992006_2.fastq.gz	Fastqsanger.gz
	SRR12992007_1.fastq.gz	Fastqsanger.gz
	SRR12992007_2.fastq.gz	Fastqsanger.gz
	SRR12992008_1.fastq.gz	Fastqsanger.gz

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RNA sequencing of single blastocyst-stage embryos (E3.5) from zygotes which were injected with sperm sRNAs from normal or depressive male mice.

Project description:We demonstrated that sperm sRNAs contribute to paternal transmission of depression-like symptoms.To further investigated the mechanism by which sperm sRNAs contribute to depression inheritance. Since the early embryonic period represents a window of plasticity important for adult phenotypes, we injected sperm sRNAs from mice of normal(F0-Ctl) or stress induced depressive(F0-Dep)into zygotes and assessed transcriptional changes when the embryos developed to the E3.5 blastocyst stage (sRNA-Dep-E3.5 vs sRNA-Ctl-E3.5).

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Genome-wide bisulfite sequencing of embryos at E4.5 stage injected with sRNAs from normal and stress-induced depressed mice

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