Project description:We report the side-effects of STZ on the spleen, and foud that the AOS10-FMT could rescure the side-effect of STZ in many factors,such as inflammation
Project description:We report the side-effects of High fat diet on the spleen, and foud that the AOS10-FMT could rescure the side-effect of High fat diet in many factors,such as inflammation
Project description:We report the side-effects of High fat diet & STZ on the spleen, and foud that the AOS10-FMT could rescure the side-effect of High fat diet & STZ in many factors,such as inflammation
Project description:Formyltransferase lacking mutants exhibit reduced growth rates in exponential phase indicating that the function of certain proteins depends on formylated N-termini but it has remained unclear, which cellular processes are abrogated by the lack of formylation. In order to elucidate how global metabolic processes are affected by the absence of formylated proteins the transcription of the fmt mutant was compared with that of the parental strain. Bacteria were grown under aerobic and anaerobic conditions and harvested in the exponential growth phase. Here we have expected the most effects of lacking formyltransferase.
Project description:The spatial organization of protein synthesis in the eukaryotic cell is essential for maintaining the integrity of the proteome and the functioning of the cell. Translation on free polysomes or on ribosomes associated with the endoplasmic reticulum has been studied for a long time. More recent data have revealed selective translation of mRNAs in other compartments, in particular at the surface of mitochondria. Although these processes have been described in many organisms, in particular in plants, the mRNA targeting and localized translation mechanisms remain poorly understood. Here, the Arabidopsis thaliana Friendly (FMT) protein is shown to be a cytosolic RNA binding protein that associates with cytosolic ribosomes at the surface of mitochondria. As previously shown (El Zawily et al., 2014), FMT knock-out delays seedling development and causes mitochondrial clustering. The mutation also disrupts the mitochondrial proteome, and the localization of nuclear transcripts encoding mitochondrial proteins at the surface of mitochondria. These data indicate that FMT participates in the localization of mRNAs and their translation at the surface of mitochondria.
