Project description:We report the side-effects of Busulfan on the heart, and foud that the AOS could rescue the side-effect of Busulfan in many factors, such as fight inflammanation, lipid metabolism and oxidative stress
Project description:The goat of this project is to exploreAlginate oligosaccharides (AOS) effect on the recovery of spleen function. RNA-seq of spleen samples from different groups: Con, busulfan(B), AOS, B+AOS.
Project description:We profiled a single-cell RNA-seq on ICR mouse testis treated with busulfan and or Alginate Oligosaccharides (AOS) from 3 weeks of age to 8 weeks of age (for 5 weeks) to study the rescue effect of AOS on the spermatogenesis disrupted by busulfan. We collected single cell from testis from the four groups AOS 0 (control), AOS 10 (AOS 10 mg/kg body weight (BW)), B+A 0 (busulfan alone), B+A 10 (busulfan plus AOS 10 mg/kg BW). The the cells were further captured with 10x Genomics based single-cell system. By using single-cell RNA seq, we found busulfan disrupted spermatogenesis by the dramatical decrease in germ cells. AOS significantly increased the number of germ cells and also promoted the germ cell development. Besides, we provide candidate master regulons regulated by AOS. Our data here offers the opportunity for treating of azoospermia or oligospermia.
Project description:We profiled a single-cell RNA-seq on ICR mouse intestine treated with busulfan and or Alginate Oligosaccharides (AOS) from 3 weeks of age to 5 weeks of age (for 2 weeks) to study the rescue effect of AOS on the intestine development disrupted by busulfan. We collected single cell from intestine from the four groups AOS 0 (control), B+A 0 (busulfan alone), B+A 10 (busulfan plus AOS 10 mg/kg Body Weight). The the cells were further captured with 10x Genomics based single-cell system. By using single-cell RNA seq, we found busulfan damaged intestinal cells. AOS significantly improved the intestinal cell junction and microvilin formation. Besides, we provide candidate master regulons regulated by AOS. Our data here offers the opportunity for improving intestine under stress.
Project description:We report the side-effects of STZ on the spleen, and foud that the AOS10-FMT could rescure the side-effect of STZ in many factors,such as inflammation
Project description:We report the side-effects of High fat diet on the spleen, and foud that the AOS10-FMT could rescure the side-effect of High fat diet in many factors,such as inflammation
Project description:We report the side-effects of High fat diet & STZ on the spleen, and foud that the AOS10-FMT could rescure the side-effect of High fat diet & STZ in many factors,such as inflammation
Project description:The aim of this project was to explore the role of Alginate oligosaccharides (AOS) on the development small intestine. The mice were treated with one dose busulfan then the mice were dosed with AOS for 5 weeks. Then the small intestine were collected. The RNA from small intestine was used for the RNA-seq analysis to search the role of AOS on small intestine development. Groups: W5A0 was from AOS 0 treatment; W5A10 was from AOS 10 treatment; W5BA0 was from busulfan and AOS 0 treatment; W5BA10 was from busulfan and AOS 10 treatment.
Project description:The goat of this project is to exploreAlginate oligosaccharides (AOS) effect on the recovery of kidney function. RNA-seq of kidney samples from different groups: Con, busulfan(B), AOS, B+AOS.
Project description:The goat of this project is to exploreAlginate oligosaccharides (AOS) effect on the recovery of heart function. RNA-seq of heart samples from different groups: Con, busulfan(B), AOS, B+AOS.
