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MiRNA expression profiling of kidney collecting duct from mice lacking Dicer1 in AQP2 expressing cells.

ABSTRACT: miRNA expression profiling of kidney collecting duct from mice lacking Dicer1 in AQP2 expressing cells.

PROVIDER: PRJNA675097 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13000002.fastq.gz	Fastqsanger.gz
	SRR13000003.fastq.gz	Fastqsanger.gz
	SRR13000004.fastq.gz	Fastqsanger.gz
	SRR13000005.fastq.gz	Fastqsanger.gz
	SRR13000006.fastq.gz	Fastqsanger.gz

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miRNA expression profiling of kidney collecting duct from mice lacking Dicer1 in AQP2 expressing cells.

Project description:To investigate the role of miRNAs in the principal cell of kidney collecting duct, a Cre-LoxP recombination strategy was used to generate a mouse model lacking the endoribonuclease Dicer in AQP2 expressing cells (DicerAQP2Cre+). To identify miRNAs altered in DicerAQP2Cre+ mice, small RNA-sequencing analysis was performed on inner medulla tissue from 1-month-old DicerAQP2Cre+ mice and controls (n=3+3 mice). Small RNA-sequencing was used as, relative to probe-based microarrays or RT-qPCR plate arrays, this approach allows both the identification of miRNAs when they have been differentially processed and it offers the possibility to identify novel miRNAs.

2021-07-14 | GSE161006 | GEO

Human iPSC-derived renal collecting duct organoids model cystogenesis in ADPKD

Project description:Here, establishing expansion cultures of hiPSC-derived ureteric bud tip cells, an embryonic precursor that gives rise to collecting ducts, we succeeded in advancing the developmental stage of collecting duct organoids and showed that all collecting duct organoids derived from PKD1-/- hiPSCs spontaneously develop multiple cysts, clarifying the initiation mechanisms of cystogenesis.

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Effects of KLF5 overexpression on renal collecting duct epithelial cell line (mIMCD-3)

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Transcription profiling of rat inner medullary collecting duct

Project description:Identification of gene expressed in the enriched inner medullary collecting duct cells in rat. Experiment Overall Design: Rat inner medullary collecting duct (IMCD) cells were isolated from 7 male Sprage-Dawley rats by collagenase and hyaluronidase digestion and follow by low speed centrifugation. The non-IMCD cells were collected by centrifugation of supernatant of enriched IMCD samples. Experiment Overall Design: Total RNA about 3 ug were used per microarray (Rat 230 2.0 Genechip array). Experiment Overall Design: The experiments were repeat 3 times (3 pairs of IMCD VS non-IMCD)

2008-06-15 | E-GEOD-7891 | biostudies-arrayexpress

Trancriptome profiling of rat inner medullary collecting duct

Project description:Identification of gene expressed in the enriched inner medullary collecting duct cells in rat. Keywords: gene expression comparision between cell type

2007-12-21 | GSE7891 | GEO

Gestational high salt stressed new born BdkrB2 knockout collecting duct cells vs BdkrB2 wild type collecting duct cells

Project description:Transcriptional profiling of new born mouse kidney collecting duct (CD) cells comparing the infuence of gestational high salt stress on gene expression remolding of BdkrB2 receptor knockout CD cells with that of BdkrB2 receptor wild type CD cells. The BdkrB2 receptor has been shown to be playing a role in renal vascular tone, kidney secretion and reabsorption function, normal kidney development, while impaired BdkrB2 receptor in kidney shown being associated with renal agenesis and renal dysplasia. Goal was to determine the effects of BdkrB2 receptor knockout together with gestational high salt stress on collecting duct gene expression pattern.

2013-08-08 | GSE49616 | GEO

Effects of KLF5 overexpression on renal collecting duct epithelial cell line (mIMCD-3)

Project description:Analysis of expression changes in renal collecting duct epithelial cells by adenoviral mediated Krüppel like transcription factor 5 (KLF5) overexpression. KLF5 is a key regulator of static and inflammatory stage in renal collecting duct epithelial cells. We thought these results provide insights into downstream genes of KLF5 in renal collecting duct epithelial cells. Total RNAs were isolated from adenovirally-mediated KLF5 over expressed cultured mIMCD-3 cells or control adenovirus infected mIMCD-3. We analyzed these two gene expression profiles after 24 hours after infection.

2011-05-17 | E-GEOD-29323 | biostudies-arrayexpress

Gestational high salt stressed new born BdkrB2 knockout collecting duct cells vs BdkrB2 wild type collecting duct cells

2013-08-08 | E-GEOD-49616 | biostudies-arrayexpress

Serine/threonine phosphatases and aquaporin-2 regulation in renal collecting duct

Project description:Phosphorylation of the aquaporin-2 (AQP2) water channel at four COOH-terminal serines plays a central role in the regulation of water permeability of the renal collecting duct. The level of phosphorylation at these sites is determined by a balance between phosphorylation by protein kinases and dephosphorylation by phosphatases. The phosphatases that dephosphorylate AQP2 have not been identified. Here, we use large-scale data integration techniques to identify serine-threonine phosphatases likely to interact with AQP2 in renal collecting duct principal cells. As a first step, we have created a comprehensive list of 38 S/T phosphatase catalytic subunits present in the mammalian genome. Then we used Bayes’ theorem to integrate available information from large-scale data sets from proteomic and transcriptomic studies in order to rank the known S/T phosphatases with regard to the likelihood that they interact with AQP2 in renal collecting duct cells. To broaden the analysis, we have generated new proteomic data (LC-MS/MS) identifying 4538 distinct proteins including 22 S/T phosphatases in cytoplasmic fractions from native inner medullary collecting duct cells from rats. The official gene symbols corresponding to the top-ranked phosphatases (common names in parentheses) were: Ppp1cb (PP1-beta), Ppm1g (PP2C), Ppp1ca (PP1-alpha), Ppp3ca (PP2-B or calcineurin), Ppp2ca (PP2A-alpha), Ppp1cc (PP1-gamma), Ppp2cb (PP2A-beta), Ppp6c (PP6C) and Ppp5c (PP5). This ranking correlates well with results of prior reductionist studies of ion and water channels in renal collecting duct cells.

2018-10-26 | PXD005488 | Pride

Gene expression analysis of collecting duct carcinoma

Project description:Gene expression analysis of collecting duct carcinoma

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