Long non-coding RNA and mRNA expression profile in the parotid gland of mouse with type 2 diabetes
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PROVIDER: PRJNA675786 | ENA |
REPOSITORIES: ENA
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Project description:To identify long non-coding RNA and mRNA expression profile in the parotid gland of mouse with type 2 diabetes, and predict the potencial role of differently expressed lncRNA in the dysfunction of diabetic PG
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Project description:To identify Circular RNA and mRNA expression profile in the parotid gland of mouse with type 2 diabetes, and predict the potential role of differently expressed circular RNA in the dysfunction of diabetic parotid gland Four 16-week-old male leptin receptor-deficient db/db mice weighting 40-55 g (a model of spontaneous type 2 DM) and age-matched db/m mice weighting 25-30 g were purchased from Changzhou Cavens Laboratory Animal Ltd. After anesthesia with an intraperitoneal injection of 1% pentobarbital sodium solution at 0.5 g/kg, the fresh PG samples were extracted and frozen with liquid nitrogen for 1-2 min, then stored at ‑80°C.
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Project description:Gene expression of 4 human parotid gland samples were comapred with 4 mouse parotid gland samples. Human and mouse parotid gland gene expression was screened using custom-designed targeted cDNA containing probes for 198 genes which encode for ion/water transporter (75 genes) and receptor/regulatory (101 genes) proteins potentially involved in the fluid secretion mechanism, as well as 11 secretory protein genes and 11 control genes
2007-08-13 | E-GEOD-6017 | biostudies-arrayexpress
Project description:As the largest salivary gland in oral cavity, the parotid gland plays an important role in initial digesting and lubricating food. The abnormal secretory function of parotid gland can lead to dental caries and oral mucosal inflammation. In recent years, single-cell RNA sequencing (scRNA-seq) has been used to explore the heterogeneity and diversity of cells in various organs and tissues. However, the transcription profile of human parotid gland at single-cell resolution has not been reported yet. In this study, we constructed the cell atlas of human parotid gland using 10x Genomics platform. Characteristic gene analysis identified the biological functions of serous acinar cell populations in secreting digestive enzymes and antibacterial proteins. We revealed the specificity and similarity of parotid gland comparing to other digestive glands through comparative analyses of other published scRNA-seq datasets. We also identified the cell-specific expression of hub genes for Sjogren’s syndrome in human parotid gland by integrating the results of GWAS and bulk RNA-seq, which highlighted the importance of immune cell dysfunction in parotid Sjogren’s syndrome pathogenesis.
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Project description:Adult parotid gland RNA-seq libraries and embryonic submandibular gland RNA-seq libraries were created to examine the mRNA species present in these secretory glands, as part of a project to understand acinar glands in general.
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Project description:By combining bulk RNA-seq and scRNA-seq approaches we have generated a comprehensive transcriptomic map of the murine parotid gland at different stages of maturation.
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Project description:Previous studies suggest that there may be age and gender related differences in salivary gland function. However, the limited and often conflicting information available from healthy populations makes it difficult to confirm these differences. The purpose of the present study was to evaluate and compare changes in gene expression associated with age and gender in the human parotid gland. Differential expression, defined as statistically significant differences with at least 1.5 fold changes, was detected using the Affymetrix® GeneChip® HGU133plus2.0 microarray in 787 gene probe sets; 320 showed higher expression in males, while 467 showed higher expression in females. Several genes associated with saliva secretion were differentially expressed in male and female parotid gland including vesicle-associated membrane protein 3 VAMP3, synaptosomal-associated protein SNAP23, RAS oncogene family member RAB1A, syntaxin binding protein STXBP1. When the gene expression results from the youngest (19-38 years old) and the oldest (65-69 years old) female subjects were further evaluated, it was found that the expression of 228 genes were altered during aging; 155 genes were down-regulated, whereas 73 genes were up-regulated in the female parotid gland. Of the genes that were altered during aging, 24 of the 28 genes (86%) classified as being associated with immune responses were down-regulated in the aged parotid gland. A panel of differentially expressed, age- and gender-related genes was selected for further study by quantitative, real-time RT-PCR. Comparable differences in gene expression were detected by both Affymetrix array and quantitative, real-time RT-PCR methods. Taken together, our data suggest that salivary gland function may be adversely affected in the aged population due, at least in part, to the down regulation of several categories of genes. Moreover, the gender specific gene expressions identified in the present study correlates with the previously observed sexual dimorphism in salivary gland function. Keywords: Human Parotid Gland
2008-12-21 | GSE8764 | GEO
Project description:Previous studies suggest that there may be age and gender related differences in salivary gland function. However, the limited and often conflicting information available from healthy populations makes it difficult to confirm these differences. The purpose of the present study was to evaluate and compare changes in gene expression associated with age and gender in the human parotid gland. Differential expression, defined as statistically significant differences with at least 1.5 fold changes, was detected using the Affymetrix® GeneChip® HGU133plus2.0 microarray in 787 gene probe sets; 320 showed higher expression in males, while 467 showed higher expression in females. Several genes associated with saliva secretion were differentially expressed in male and female parotid gland including vesicle-associated membrane protein 3 VAMP3, synaptosomal-associated protein SNAP23, RAS oncogene family member RAB1A, syntaxin binding protein STXBP1. When the gene expression results from the youngest (19-38 years old) and the oldest (65-69 years old) female subjects were further evaluated, it was found that the expression of 228 genes were altered during aging; 155 genes were down-regulated, whereas 73 genes were up-regulated in the female parotid gland. Of the genes that were altered during aging, 24 of the 28 genes (86%) classified as being associated with immune responses were down-regulated in the aged parotid gland. A panel of differentially expressed, age- and gender-related genes was selected for further study by quantitative, real-time RT-PCR. Comparable differences in gene expression were detected by both Affymetrix array and quantitative, real-time RT-PCR methods. Taken together, our data suggest that salivary gland function may be adversely affected in the aged population due, at least in part, to the down regulation of several categories of genes. Moreover, the gender specific gene expressions identified in the present study correlates with the previously observed sexual dimorphism in salivary gland function. Experiment Overall Design: Human parotid glands were obtained from healthy male and female subjects (19-85 years of age). 3 young female parotid glands were compared with 3 older female parotid gland. 8 Female samples were compared with 5 male samples
2009-01-13 | E-GEOD-8764 | biostudies-arrayexpress
Project description:Identification of circRNA expression profiles and functional networks in parotid gland of type 2 diabetes mouse
| PRJNA976667 | ENA