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Transcriptome sequencing (RNA-Seq) of kidney and liver tissues from Lxrα-/- mices or wild type mices.

ABSTRACT: Transcriptome sequencing (RNA-Seq) of kidney and liver tissues from Lxrα-/- mices or wild type mices.

PROVIDER: PRJNA679777 | ENA |

REPOSITORIES: ENA

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Transcriptome sequencing (RNA-Seq) of kidney and liver tissues from Lxrα-/- mices or wild type mices.

Project description:As a cholesterol sensor, nuclear receptor liver X receptor (Lxr) activated by oxysterols is an important factor regulating cholesterol homeostasis and plays central roles in the transcriptional control of cholesterol metabolism. Lxrα is one of the liver X receptor(Lxrs) subtypes. Lxrα is abundantly expressed in liver, small intestine, adipose, kidney, adrenal tissues, and macrophages. Lxrα's regulation of lipid metabolism is well known but its regulation of other downstream genes remains unclear.In order to investigate the regulating effect of Lxrα on other genes, we used the second-generation transcriptome sequencing(RNA-seq) to determine the expression changes of each gene in Lxrα-/- mices comparing with wild type mices.

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Transcriptome sequencing (RNA-Seq) of liver tissues from LXRα-/- mices and wild type mices fed with a chow diet or a high fat diet (HFD).

Project description:Liver X receptor α (LXRα） is a key regulator of lipid homeostasis and effective inhibitors of inflammation. We used the second-generation transcriptome sequencing(RNA-seq) to determine the expression changes of each gene in LXRα-/- mices and wild type mices fed with a chow diet or a high fat diet (HFD).We investigated the downstream molecular mechanisms by which loss of LXRα regulates lipid synthesis and metabolism in HFD.

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Next Generation Sequencing of Wild Type and LXRα-Ser196 phosphorylation deficient Murine Hepatic Transcriptomes on a Chow diet

Project description:Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess changes in the hepatic transcriptional profiles of mice that carry a whole-body phosphorylation deficient knock in mutant of LXRα (S196A) compared to wild-type (WT) fed a chow diet.

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Next Generation Sequencing of Wild Type and LXRα-Ser196 phosphorylation deficient Murine Hepatic Transcriptomes on a High Fat/High Cholesterol Diet

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Transcription profiling of mouse brain, kidney, stomach, liver, and salivary tissues of perinatal ERRI? null and wild type mice ERRIalpha Regulates Cardiac, Gastric and Renal Potassium Homeostasis

Project description:Genomic analysis of expression in brain, kidney, stomach, liver, and salivary tissues of perinatal ERRÎ³ null and wild type mice. Experiment Overall Design: Total RNA was extracted from brain, kidney, stomach, liver, and salivary tissues of perinatal ERRÎ³ null and wild type mice and hybridized to Affymetrix MOE430A gene chips in triplicate.

2009-11-14 | E-GEOD-18896 | biostudies-arrayexpress

Transcription profiling of intestine, kidney and liver tissues from adult male knock-out mice for all Cyp3a genes versus wild type mice

Project description:Comparision of gene expression profiles of intestine, kidney and liver tissues of adult male knock-out mice for all Cyp3a genes (Cyp3a-/- genotype) versus adult male wild-type control mice (FVB genotype).

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Foam cell specific LXRα ligand

Project description:OBJECTIVE: The liver X receptor α (LXRα) is a ligand-dependent nuclear receptor and the major regulator of reverse cholesterol transport in macrophages. This makes it an interesting target for mechanistic study and treatment of atherosclerosis. METHODS AND RESULTS: We optimized a promising stilbenoid structure (STX4) in order to reach nanomolar effective concentrations in LXRα reporter-gene assays. STX4 displayed the unique property to activate LXRα effectively but not its subtype LXRβ. The potential of STX4 to increase transcriptional activity as an LXRα ligand was tested with gene expression analyses in THP1-derived human macrophages and oxLDL-loaded human foam cells. Only in foam cells but not in macrophage cells STX4 treatment showed athero-protective effects with similar potency as the synthetic LXR ligand T0901317 (T09). Surprisingly, combinatorial treatment with STX4 and T09 resulted in an additive effect on reporter-gene activation and target gene expression. In physiological tests the cellular content of total and esterified cholesterol was significantly reduced by STX4 without the undesirable increase in triglyceride levels as observed for T09. CONCLUSIONS: STX4 is a new LXRα-ligand to study transcriptional regulation of anti-atherogenic processes in cell or ex vivo models, and provides a promising lead structure for pharmaceutical development.

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Next Generation Sequencing of LdlrKO LXRα-phosphorylation disrupted macrophage transcriptomes

Project description:Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. Besides ligand binding, LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess changes in bone marrow derived macrophage transcriptional profiles of mice that carry LysMcre directed phosphorylation deficient-version of LXRα compared (S196A) to wild-type (WT).

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2023-10-01 | GSE185142 | GEO

TLR-dependent transcriptional reprogramming of LXRα cistrome in bone marrow-derived macrophages

Project description:The Liver X receptors (LXRα and LXRβ) are members of the nuclear receptor superfamily of transcription factors, and play key roles in the coordination of both metabolic and immune responses in macrophages. Both LXRs are expressed in macrophages and they share a high degree of sequence homology but their individual roles in different models of macrophage cultures, particularly in response to inflammation, have not been characterized. Previous studies have shown that ligand pre-treated LXRs repress the induction of pro-inflammatory cytokines in macrophages. Here, we show evidence that the expression of LXRα (but not LXRβ) is transcriptionally regulated at late phases of an LPS-induced inflammatory response in bone marrow-derived macrophages (BMDMs). Regulation of LXRα expression is controlled at the transcriptional levels by inflammatory signaling networks. Transcriptional profiling studies and ChIP-seq data identified a number of innate immune pathways whose expression is substantially controlled by LXRα in BMDMs. In summary, our data shows that LXRα plays a previously unrecognized role in innate immunity by transcriptional regulation of a battery of inflammatory genes through TLR and Type-I IFN-dependent signaling in macrophages.

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