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Single cell RNA-seq analysis on human myometrium and leiomyomas

ABSTRACT: Single cell RNA-seq analysis on human myometrium and leiomyomas

PROVIDER: PRJNA680734 | ENA |

REPOSITORIES: ENA

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Single cell RNA-seq analysis on human myometrium and leiomyomas

Project description:To understand the cellular and molecular heterogeneity in the normal myometrium, we performed single-cell RNA sequencing on normal myometrium samples. We discovered 17 different cell lineages in the normal myometrium. Our work provides a single cell atlas for the normal myometrium.

2022-08-27 | GSE162122 | GEO

Comparison of Gene expression profiles between myometrium, MED12 mutation positive uterine leiomyomas and MED12 wildtype uterine leiomyomas

Project description:Gene expression profiles of 10 uterine leiomyomas and their matched normal myometrium specimens were studied using Affymetrix GeneChip Human Genome U133 Plus 2.0 gene expression arrays. Four tumors displayed a codon 44 mutation, four carried a intron 1 mutation, and the remaining two displayed no MED12 mutation.

2011-08-26 | GSE30673 | GEO

Epigenome analysis of uterin leiomyoma and myometrium with or without leiomyoma [Methylation data]

Project description:Profiles of genome-wide DNA methylation were investigated in leiomyomas and in myometrium with and without leiomyomas. Profiles of DNA methylation in the myometrium with and without leiomyomas were quite similar while those in leiomyomas were distinct. Bisulphite converted DNA from the three uterine leiomyoma, three myometrium with leiomyoma and three myometrium without leiomyoma were hybridised to the Illumina infinium HumanMethylation450 BeadChip.

2013-09-01 | E-GEOD-45187 | biostudies-arrayexpress

Epigenome analysis of uterin leiomyoma and myometrium with or without leiomyoma [Expression data]

Project description:Profiles of genome-wide DNA methylation were investigated in leiomyomas and in myometrium with and without leiomyomas. Profiles of DNA methylation in the myometrium with and without leiomyomas were quite similar while those in leiomyomas were distinct. Total RNA from the three uterine leiomyoma, three myometrium with leiomyoma and three myometrium without leiomyoma were analyzed with the Affymetrix GeneChip Mouse Gene 1.0 ST Array.

2013-09-01 | E-GEOD-45188 | biostudies-arrayexpress

Epigenome analysis of uterin leiomyoma and myometrium with or without leiomyoma [Expression data]

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Epigenome analysis of uterin leiomyoma and myometrium with or without leiomyoma [Methylation data]

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Analysis of the purified RNA samples to confirm that Notch pathway activation inhibits apoptosis in uterine leiomyomas leading to fibroid growth

Project description:Uterine leiomyomas are the most common benign tumor in humans causing significant morbidity with vaginal bleeding, pelvic pressure and pain. Histologically, leiomyomas show a large degree of extracellular matrix disorganization. I am working with a colleague who recently found Notch pathway gene expression were clearly altered in fibroids (“Differential expression of the Notch signal transduction pathway: ligands, receptors and Numb in uterine leiomyomas vs. myometrium,” G. Christman, H. Tang, I. Ahmad, J. Stribley, Fertility and Sterility, Volume 88, Supp 1, S72, September 2007). Glycosaminoglycan expression was found to be over-expressed in uterine leiomyomas compared to myometrial samples (Fertility and Sterility, Vol 88 Supp 1, S106, September, 2007), but glycosyltransferase and glycosidase expression has not been reported. We have purified RNA samples from paired uterine leiomyoma and normal myometrium from a previous clinical study. Dr. Domino's laboratory hypothesis is that Notch pathway activation inhibits apoptosis in uterine leiomyomas leading to fibroid growth. Notch ligands are fucosylated glycans. The bulk of a fibroid is the extracellular matrix yet little has been studied on leiomyocyte expression of enzymes that model glycans in the extracellular matrix. RNA preparations of paired samples of excess human tissues from hysterectomies, with two different groups normal myometrium, and leiomyoma tumors were sent to Microarray Core (E). The RNA was amplified, labeled, and hybridized to GLYCO_v3 microarrays.

2011-04-29 | E-GEOD-28945 | biostudies-arrayexpress

Differential Expression of MED12-Associated Coding RNA 2 transcripts in Uterine Leiomyomas

Project description:The objective of this study was to elucidate the expression profile of coding RNA transcripts in leiomyomas with and without MED12 mutation and their paired myometrium.

2023-03-01 | GSE224991 | GEO

Analysis of the purified RNA samples to confirm that Notch pathway activation inhibits apoptosis in uterine leiomyomas leading to fibroid growth

2011-04-29 | GSE28945 | GEO

Profiling of Myometrium and Leiomyoma transcriptomes in 15 leiomyoma (MED12 G44 mutant) and 15 matched normal myometrium (WT) patient tissue samples.

Project description:Through comprehensive profiling of transcribed genes in myometrium and leiomyoma tissue samples, we demonstrate two distinct gene expression profiles for myometrium and leiomyoma. This study shows that transcriptional dysregulation, and not post-transcriptional dysregulation, is primarily responsible for the perturbations of key biological processes in leiomyomas such as the extracellular matrix organization.

2020-01-01 | GSE128229 | GEO

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