Project description:Transcriptional profiling of Caco-2 cells co-cultured with L. fermentum AGR1487 (isolated from IBD patient), Caco-2 cells co-cultured with L. fermentum AGR1485 (isolated from healthy volunteer), or Caco-2 cells alone (Control).
Project description:Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours.
Project description:ApoM may be a crucial regulator of colorectal cancer progression, but its role in colorectal cancer has not been elucidated. Bioinformatic analysis of the microarray data revealed unique transcriptomes under different apoM expression. We used microarrays to detail the global programme of gene expression underlying different apoM genetypes and identified distinct classes of regulated genes
Project description:Caco-2 cells (intestinal human derived cell line) were cultured on transwell plates, and exposed to a stimulus (IFNg) through the bottom compartment of the transwell plates
Project description:Transcriptional profiling of Caco-2 cells co-cultured with Faecalibacterium prausnitzii DSM17677, Lactobacilus rhamnosus HN001, UV-killed F. prausnitzii, or no bacteria in an apical anaerobic environment for four hours.
Project description:To compare gene expression in human intestinal cell line after Sirt1 manipulation by overexpression or targetted siRNA knockdown Triplicate biological replicates for Caco-2 control and Sirt1 overexpression (6 samples), duplicate biological replicates for Caco-2 control siRNA and two non-overlapping targetted Sirt1 siRNAs (6 samples)
Project description:Transcriptional profiling of Caco-2 cells co-cultured with L. fermentum AGR1487 (isolated from IBD patient), Caco-2 cells co-cultured with L. fermentum AGR1485 (isolated from healthy volunteer), or Caco-2 cells alone (Control). Three-condition experiment with dye swap, A vs B, A vs C, B vs C, 8 biological replicates: A = Control, B = AGR1487, C = AGR1485
Project description:Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours. Two-condition experiment with dye swap, A vs B, 6 biological replicates
Project description:Effect of Lactobacillus plantarum MB452 on the gene expression of the intestinal epithelial cell line Caco-2 using a reference design Experiment Overall Design: Effect of Lactobacillus plantarum MB452 on the gene expression of the intestinal epithelial cell line Caco-2 using a reference design
Project description:Whole genome microarray data were analyzed to describe the changes in gene transcription profile in human Caco-2 cancer cells under the influence of the extract from iodine-biofortified and non-fortified carrot and lettuce. These iodine-biofortified vegetables can be used as a functional food. Four-condition experiment: iodine-biofortified carrot, non-fortified carrot, iodine-biofortified lettuce, non-fortified lettuce vs. Caco-2 colorectal adenocarcinoma cell line. Three biological replicates and three technical replicates.