Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNA profiles on hESC-derived lung cells and lung-macrophage co-cultured cells

ABSTRACT: scRNA profiles on hESC-derived lung cells and lung-macrophage co-cultured cells

PROVIDER: PRJNA684305 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13238783_1.fastq.gz	Fastqsanger.gz
	SRR13238783_2.fastq.gz	Fastqsanger.gz
	SRR13238784_1.fastq.gz	Fastqsanger.gz
	SRR13238784_2.fastq.gz	Fastqsanger.gz
	SRR13238785_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

scRNA profiles on hESC-derived lung cells and lung-macrophage co-cultured cells

Project description:We established a co-culture model using cells derived from hPSC and studied the transcriptomes on the lung cells and the co-cultures using scRNA-seq.

2022-03-14 | GSE162996 | GEO

scRNA profiles on hESC-derived lung cells

Project description:scRNA profiles on hESC-derived lung cells

| PRJNA683877 | ENA

scRNA profiles on hESC-derived lung cells

Project description:Study transcriptomes on the lung cells derived from hESC at day50

2020-12-16 | GSE162936 | GEO

Ascorbate effect on serum free cultured hESC

Project description:Ascorbate activates CD30 expression and causes widespread specific demethylation of the epigenome of serum free cultured hESC. The genetic and epigenetic integrity of human Embryonic Stem cells (hESCs) is critical to their future applications in research and medicine. hESC cultured in serum free media can accumulate point mutations, aneuploidy and progressive epigenetic changes over prolonged culture in vitro. We have identified ascorbate as the only molecule in the very widely used Knock-out serum replacement medium that is sufficient to induce expression of CD30, a biomarker for aneuploidy in hESCs. In fact, we show that hESC cultured in the presence of ascorbate for 20 passages not only display demethylation of the CD30 locus, but exhibit widespread and remarkably specific and consistent demethylation of 1,847 genes in both HES2 and HES3 cells. The specific ascorbate induced demethylation changes in the hESC epigenome, of which 86% are shared between the two lines, identify a subset of genes in hESC, including CD30, that are sensitive to serum free culture medium induced epigenetic changes. Experiment. HES2 cells were maintained on 20% Fetal calf serum on mouse embryonic fibroblast feeder layers with mechanical dissection. Using HES2 after 99 mechanical passages (P99), hESC were grown for 17-20 (+17 - +20) passages in the presence of 20% Knock-Out Serum Replacement (Invitrogen) either with (+ASC) or without (-ASC) Ascorbate with enzymatic culturing.

2010-06-10 | GSE16590 | GEO

Ascorbate effect on serum free cultured hESC

2010-06-23 | E-GEOD-16590 | biostudies-arrayexpress

Profiling of cultured primary stromal cells from human lung

Project description:Profiling of cultured primary stromal cells from human lung

| PRJNA699012 | ENA

Transcriptome analysis of primary airway epithelial cells co-cultured with human lung fibroblasts

Project description:Transcriptome analysis of primary airway epithelial cells co-cultured with human lung fibroblasts

| PRJNA846269 | ENA

TMT characterisation: iPSC vs hESC

Project description:Detailed comparison of human pluripotent stem cells, to determine how hESC and iPSC differ in their protein expression profiles. The data was acquired in a TMT 10-plex SPS-MS3. 4 replicates of hESC compared to 4 replicates of HipSci human iPSCs.

2021-11-03 | PXD014502 | Pride

Expression of four hESC lines in enzymatic and mechanic passaging methods

Project description:hESC have morphologic, genetic and genomic alternatiions when cells cultured in different passaging condition. Here transcriptome of four different hESC lines were compared in two passaging methods.

2015-06-01 | GSE47916 | GEO

Gene expression of human cancer cells co-cultured with mouse astrocytes and fibroblasts after series of cross co-culture

Project description:Brain metastases are highly resistant to chemotherapy. Brain metastases are surrounded and infiltrated by activated astrocytes. To examine the genes whose expression is associated with chemo-resistance of brain-metastasized cancer cells, gene expression data were collected and analyzed from breast cancer cells and lung cancer cells co-cultured with astrocytes. Fibroblast cells were used as control. Human lung cancer cell PC14 was co-cultured with mouse astrocytes or fibroblasts for two rounds. Total RNAs were extracted from co-cultured cells and hybridized to human microarray.

2012-12-31 | E-GEOD-26292 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data