Project description:The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective fashion. Here, we combine Hi-C data with existing draft assemblies to generate chromosome-length scaffolds. We validate this method by assembling a human genome, de novo, from short reads alone (67X coverage, Sample GSM1551550). We then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors Aedes aegypti and Culex quinquefasciatus, each consisting of three scaffolds corresponding to the three chromosomes in each species. These assemblies indicate that virtually all genomic rearrangements among these species occur within, rather than between, chromosome arms. The genome assembly procedure we describe is fast, inexpensive, accurate, and can be applied to many species.
Project description:The draft genome of L. sativa (lettuce) cv. Tizian was sequenced in two Illumina sequencing runs, mate pair and shotgun. This entry contains the RAW sequencing data.
Project description:Plutella xylostella is the major cosmopolitan pest of brassica and other crucifer crops,the larval midgut of which is a dynamic organ that interfaces with a diversearray of physiological and toxicological processes.The draft sequence of the P.xylostella genome was recently released,but its annotation remains chanllenging because of the low coverage of this branch of life.Peptide sequencing by computational assignment of tandem mass spectra to a database of putative protein sequences provides an independent approach to confirm or refute protein prediction,which has been termed proteogenomics.In this study,we carried out an in-depth proteogenomic analysis using shotgun HPLC-ESI-MS/MS approach with a multi-algorithme peipline to complement genome annotation in the P.xylostella larval midgut.
Project description:Plutella xylostella is the major cosmopolitan pest of brassica and other crucifer crops,the larval midgut of which is a dynamic organ that interfaces with a diversearray of physiological and toxicological processes.The draft sequence of the P.xylostella genome was recently released,but its annotation remains chanllenging because of the low coverage of this branch of life.Peptide sequencing by computational assignment of tandem mass spectra to a database of putative protein sequences provides an independent approach to confirm or refute protein prediction,which has been termed proteogenomics.In this study,we carried out an in-depth proteogenomic analysis using shotgun HPLC-ESI-MS/MS approach with a multi-algorithme peipline to complement genome annotation in the P.xylostella larval midgut.
Project description:Plutella xylostella is the major cosmopolitan pest of brassica and other crucifer crops,the larval midgut of which is a dynamic organ that interfaces with a diversearray of physiological and toxicological processes.The draft sequence of the P.xylostella genome was recently released,but its annotation remains chanllenging because of the low coverage of this branch of life.Peptide sequencing by computational assignment of tandem mass spectra to a database of putative protein sequences provides an independent approach to confirm or refute protein prediction,which has been termed proteogenomics.In this study,we carried out an in-depth proteogenomic analysis using shotgun HPLC-ESI-MS/MS approach with a multi-algorithme peipline to complement genome annotation in the P.xylostella larval midgut.
Project description:Plutella xylostella is the major cosmopolitan pest of brassica and other crucifer crops,the larval midgut of which is a dynamic organ that interfaces with a diversearray of physiological and toxicological processes.The draft sequence of the P.xylostella genome was recently released,but its annotation remains chanllenging because of the low coverage of this branch of life.Peptide sequencing by computational assignment of tandem mass spectra to a database of putative protein sequences provides an independent approach to confirm or refute protein prediction,which has been termed proteogenomics.In this study,we carried out an in-depth proteogenomic analysis using shotgun HPLC-ESI-MS/MS approach with a multi-algorithme peipline to complement genome annotation in the P.xylostella larval midgut.
