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Trx knockdown in intestinal stem cells (ISCs) of Drosophila melanogaster

ABSTRACT: Trx knockdown in intestinal stem cells (ISCs) of Drosophila melanogaster

PROVIDER: PRJNA686011 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13267288.fastq.gz	Fastqsanger.gz
	SRR13267289.fastq.gz	Fastqsanger.gz
	SRR13267290.fastq.gz	Fastqsanger.gz
	SRR13267291.fastq.gz	Fastqsanger.gz

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Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons [RNA-seq trx]

Project description:We have analyzed changes in histone H3 lysine 4 methylation (H3K4me) and gene expression resulting from trx knockdown (KD) in adult mushroom body neurons of Drosophila melanogaster.

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Trx knockdown in intestinal stem cells (ISCs) of Drosophila melanogaster

Project description:Male flies were crossed to virgins of genotype yw;esg-Gal4,UAS-GFP;tub-Gal80ts/Tm3,Sb. Progeny was collected and aged 3-4 days before shifting for 7 days to 29 degrees to induce RNAi expression. Guts were dissected for 2 biological replicates/condition, each containing 80-100 guts, dissociated and GFP-positive cells were FACS-sorted. RNA was isolated using the PicoPure RNA-isolation kit and libraries were generated. Sequencing was done by distributing the 4 libraries over 5 lanes on the Illumina HiSeq2000 platform.

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trx regulatory gene network in D. melanogaster

Project description:This SuperSeries is composed of the following subset Series: GSE8748: Chromosomal clustering of genes in trithorax (trx) mutant larvae GSE8750: Chromosomal clustering of genes in absent, small, or homeotic discs 2 (ash2) mutant larvae Keywords: SuperSeries Refer to individual Series

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Project description:Drosophila melanogaster nxf2 knockdown

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An epigenetic mark of polycomb response elements implemented by Trx/MLL/COMPASS

Project description:In Drosophila, Polycomb Response Elements (PREs) are identified as genomic sequences allowing the maintenance of transcriptional repression in the absence of the initiating signal. Although PREs in Drosophila are well characterized, the existence of mammalian PRE-like elements remains debated. Accumulating evidence supports a model in which CpG islands function to recruit Polycomb-Group complexes (PcG), however, it is not evident which subclasses of CpG islands serve as PREs. Trithorax (Trx), which is required for positive regulation of gene expression in Drosophila, is known to co-bind Drosophila PREs where it is thought to antagonize polycomb-dependent silencing of nearby genes. Here, we demonstrate the existence of Trx-dependent H3K4 dimethylation loci that specifically mark Drosophila PREs and are required for the maintenance of expression of the nearby genes. Similarly, in human cells, we find ~ 3000 MLL1 (human Trx homologue)-dependent H3K4 dimethylation loci, which correlate strongly with CpG island density. In the absence of MLL1 and H3K4 dimethylation at these loci, there is an increase in H3K27 trimethylation levels, suggesting these sites can recruit Polycomb Repressive Complex 2 (PRC2). By inhibiting PRC2-dependent silencing in the absence of MLL1, we establish that a balance exists between MLL1 and PRC2, and their respective capacity to maintain or repress transcription. Thus, by investigating a conserved function between Trx and MLL1, we provide rules for the identification of CpG island subclasses serving as PRE-like sequences within the human genome. To examine changes in histone-modification profiles and gene expression after depletion of Trx in Drosophila S2 cells, and MLL1 in human HCT116 cells. We also treated MLL1-NULL HCT116 cells with GSK126 (5uM) for 4 days and measured changes in gene expression.

2016-07-26 | E-GEOD-81795 | biostudies-arrayexpress

RNAseq of Aging Drosophila ISCs

Project description:RNAseq of Aging Drosophila ISCs

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Chromosomal clustering of genes in trithorax (trx) mutant larvae

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2008-09-26 | GSE8748 | GEO

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