MRNA expression data in epididymal fat tissues derived from mir342-/- and mir342wt/wt mice
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PROVIDER: PRJNA688062 | ENA |
REPOSITORIES: ENA
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Project description:mir342-/- and mir342wt/wt C57BL/6J mice were fed with standard diet (STD) and high fat-high sucrose diet (HFHS). We used microarrays to investigate the mRNA expression profile in the epididymal adipose tissues.
2020-12-28 | GSE163880 | GEO
Project description:mir221/222 AdipoKO and mir221/222 flox/y C57BL/6J mice were fed with standard diet (STD) and high fat-high sucrose diet (HFHS). We used microarrays to investigate the mRNA expression profile in the epididymal adipose tissues.
2020-12-29 | GSE163921 | GEO
Project description:Analysis of gene expression profiles of epididymal fat from DIO rats We applied a comparative functional genomics approach to evaluate diet-induced obese (DIO) rats as an obesity model Keywords: single time point, comparison control animal vs. diet induced obese animal
2007-08-07 | GSE8700 | GEO
Project description:Comparison of gene expression profiles from epididymal and retroperitoneal fat in adult male SD rats.
2009-05-30 | E-MEXP-1993 | biostudies-arrayexpress
Project description:The QTL for fatty liver was mapped on mouse chromosome 12, designated as Fl1sa. Iah1 gene is a candidate gene for Fl1sa . In mammal, Iah1 function has been largely unknown. We constructed Iah1-knockout mouse in A/J-12SM background. Iah1 gene is abundantly expressed in epididymal fat of mouse. We compared the epididymal fat's gene expressions between Iah1-WT mouse and Iah1-KO mouse.
2020-03-19 | GSE145750 | GEO
Project description:Dietary administration of n-3 polyunsaturated fatty acids (PUFA) is often associated with altered adipose tissue (AT) morphology and/or function in obese mice. However, it is unclear whether these observations are an indirect consequence of reduced weight gain or result from direct actions of n-3 PUFA. Here we studied the AT of fat-1 transgenic mice that convert endogenous n-6 to n-3 PUFA. These mice display equivalent weight gain and fat accretion to their wild-type (WT) counterparts. We performed Affymetrix microarray in epididymal AT of male hemizygous fat-1(+/-) transgenic mice and their wild type littermates that had been fed high fat diets from 6 weeks of age (diet-93075, 55% Kcal from fat, Harlan Teklad). Mice were sacrificed after 8 weeks on the HF diet. At sacrifice epididymal adipose tissues excised for the microarray study (n=3 per group) were rapidly homogenized in QIAZOL (QIAGEN) and snap frozen in liquid nitrogen.
2015-02-06 | E-MTAB-1831 | biostudies-arrayexpress
Project description:Analysis of gene expression profiles of epididymal fat from DIO rats; We applied a comparative functional genomics approach to evaluate diet-induced obese (DIO) rats as an obesity model Experiment Overall Design: Gene expression profiles from DIO and lean rats were generated and compared. A private colony of male Long-Evans rats was set up at Harlan (Harlan-Sprague Dawley, Indianapolis, IN). The animals had been on either a high fat/high sucrose diet (TD95217, Harlan Takled, Madison, WI) or a regular chow (Purina 5008; Ralston-Purina, St. Louis, MO) since weaning. Rats were shipped at 14-weeks of age to our facility and maintained on a 12:12-hour light-dark photoperiod (light on 10:00 PM). They were individually housed throughout the study at ambient temperature, and had free access to diets and water. After 2 weeks acclimation to the facility, body weight of the rats and weight of food consumed in the last 7 days were measured once a week. Body fat mass was analyzed by nuclear magnetic resonance (NMR) using an Echo Medical System (Houston, TX) instrument 3 times throughout the study. Fat free mass is the difference between body mass and fat mass. All animal studies were conducted in compliance with approved institutional animal care and use protocols according to NIH guidelines (NIH publication No. 86-23, 1985). OGTT was conducted after an overnight fasting when animals were 21 weeks of age. After a baseline blood collection (time 0), animals were dosed orally with 1.5 gram/kg glucose. Subsequence blood samples were collected at 30, 60, and 120 minutes post glucose challenge for the determination of plasma glucose and insulin levels. At 25 weeks of age, blood was collected by tail bleeding after overnight fasting. Rats were killed a week later at 09:00am by rapid decapitation. Trunk blood was collected and epididymal fat tissue excised. Various circulating metabolic parameters were measured under fasted (week 25) and fed conditions (week 26). Total RNA from rat epididymal fat was isolated with RNA STAT-60 (Tel-Test) according to the manufacturer's protocol. 5 micrograms of total RNA were labeled and hybridized to Affymetrix RAE230_2 arrays according to the Affymetrix protocol.
2009-04-23 | E-GEOD-8700 | biostudies-arrayexpress
Project description:Pooled RNA samples were prepared for each of the four mouse strainsDU6, DU6i, DUKs, and DBA by epididymal fat tissues of 42 days old 20 male per lines. Pooling was used to reduce individual variability, which is high in outbred populations. For the hybridization of the tissue specific RNAs to the GeneChips, samples were prepared according to the recommendations of the Affymetrix user guide. First strand synthesis was carried out by a T7-(dT)24 primer and SuperScript II Reverse Transcriptase (Gibco BRL, Life Technologies GmbH, Eggenstein, Germany) using 10 mg whole RNA sample. Second strand synthesis was done according to the SuperScript Choice System (Gibco BRL, Life Technologies GmbH) by E. coli DNA-Polymerase I, E. coli Ligase and RNaseH. Fragment end-polishing was performed using T4-Polymerase. An in vitro transcription reaction was used to incorporate Biotin-11-CTP and Biotin-16-UTP to the cRNA probe (BioArray HighYield RNA Transcript Labeling Kit, Enzo). The fragmented cDNA was hybridized overnight at 45°C to ensure the quality of the probe. The expression levels were calculated with the GeneChip Expression Analysis softwares Data Mining Tool (Version 1.2.) and Microarray Suite (version 4.0.1.) provided by Affymetrix. Initially, hybridization signals on every GeneChip were scaled using all probe sets to minimize differences in overall signal intensities between two arrays allowing more reliable detection of biologically relevant changes in the sample. The Affymetrix decision matrix was used for the assessment of present, marginally present, and absent transcript amounts of a gene in a tested RNA. Keywords = adiposity Keywords = selected mouse lines Keywords = diabetes Keywords = insulin Keywords = leptin Keywords: parallel sample
2004-11-09 | GSE1936 | GEO
Project description:Pooled RNA samples were prepared for each of the four mouse strainsDU6, DU6i, DUKs, and DBA by epididymal fat tissues of 42 days old 20 male per lines. Pooling was used to reduce individual variability, which is high in outbred populations. For the hybridization of the tissue specific RNAs to the GeneChips, samples were prepared according to the recommendations of the Affymetrix user guide. First strand synthesis was carried out by a T7-(dT)24 primer and SuperScript II Reverse Transcriptase (Gibco BRL, Life Technologies GmbH, Eggenstein, Germany) using 10 mg whole RNA sample. Second strand synthesis was done according to the SuperScript Choice System (Gibco BRL, Life Technologies GmbH) by E. coli DNA-Polymerase I, E. coli Ligase and RNaseH. Fragment end-polishing was performed using T4-Polymerase. An in vitro transcription reaction was used to incorporate Biotin-11-CTP and Biotin-16-UTP to the cRNA probe (BioArray HighYield RNA Transcript Labeling Kit, Enzo). The fragmented cDNA was hybridized overnight at 45°C to ensure the quality of the probe. The expression levels were calculated with the GeneChip Expression Analysis softwares Data Mining Tool (Version 1.2.) and Microarray Suite (version 4.0.1.) provided by Affymetrix. Initially, hybridization signals on every GeneChip were scaled using all probe sets to minimize differences in overall signal intensities between two arrays allowing more reliable detection of biologically relevant changes in the sample. The Affymetrix decision matrix was used for the assessment of present, marginally present, and absent transcript amounts of a gene in a tested RNA.
2008-06-11 | E-GEOD-1936 | biostudies-arrayexpress
Project description:mRNA expression data in epididymal fat tissues derived from mir221/222 adipose tissue specific knockout (AdipoKO) and mir221/222 flox/y mice
| PRJNA688220 | ENA