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Transcriptome differences in FCRL5 positive and negative Influenza-specific memory B cells

ABSTRACT: Transcriptome differences in FCRL5 positive and negative Influenza-specific memory B cells

PROVIDER: PRJNA688481 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13324200_1.fastq.gz	Fastqsanger.gz
	SRR13324200_2.fastq.gz	Fastqsanger.gz
	SRR13324300_1.fastq.gz	Fastqsanger.gz
	SRR13324300_2.fastq.gz	Fastqsanger.gz
	SRR13324201_1.fastq.gz	Fastqsanger.gz

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Transcriptome differences in FCRL5 positive and negative Influenza-specific memory B cells

Project description:We examined the transcriptional differences between circulating B cell subsets post-vaccination. Using FCRL5 as a marker of Tbet expressing B cells, we sorted FCRL5+ (Tbet+) and FCRL5- (Tbet-) memory B cells, naïve B cells, and antibody secreting plasmablasts at day 7 and day 14 post-vaccination. Additionally, we used H1 B cell tetramers to track cells responding to the influenza vaccine. These data provide insights into the transcriptional programming of distinct B cell subsets that respond to influenza vaccination.

2023-03-01 | GSE163989 | GEO

Accessible chromatin differences in FCRL5 positive and negative influenza-specific memory B cells

Project description:We examined the accessible chromatin differences between circulating B cell subsets post-vaccination. Using FCRL5 as a marker of Tbet expressing B cells, we sorted FCRL5+ (Tbet+) and FCRL5- (Tbet-) memory B cells, and antibody secreting plasmablasts at day 7 and day 14 post-vaccination. Additionally, we used H1 B cell tetramers to track cells responding to the influenza vaccine in a subset of samples. These data provide insights into the accessible chromatin landscape of distinct B cell subsets that respond to influenza vaccination.

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Accessible chromatin differences in FCRL5 positive and negative influenza-specific memory B cells

Project description:Accessible chromatin differences in FCRL5 positive and negative influenza-specific memory B cells

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The memory B cell response to influenza vaccination is impaired in older persons

Project description:Influenza imparts an age-related increase in mortality and morbidity. The most effective countermeasure is vaccination; however, vaccines offer modest protection in older adults. To investigate how ageing impacts the memory B cell response we tracked haemagglutinin specific B cells by indexed flow sorting and single cell RNA sequencing in twenty healthy adults administered the trivalent influenza vaccine. We found age-related skewing in the memory B cell compartment six weeks after vaccination, with younger adults developing haemagglutinin specific memory B cells with an FCRL5+ “atypical” phenotype, showing evidence of somatic hypermutation and positive selection, which happened to a lesser extent in older persons. We confirmed the germinal center ancestry of these FCRL5+ “atypical” memory B cells using scRNASeq from fine needle aspirates of influenza responding human lymph nodes and paired blood samples. Together, this study shows that the aged human germinal center reaction and memory B cell response following vaccination is defective.

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Expansion of an FCRL5+ B cell subset resembling atypical memory B cells in an animal model of malaria

Project description:Background: In human malaria, parasites of the genus Plasmodium elicit expansion of atypical memory B cells (atMBCs), which lack the classical markers CD21 and CD27. We have identified a putative population of analogous B cells in a murine model of infection with P. chabaudi, delineated by the marker FCRL5. We performed RNA-Seq on FCRL5+ and FCRL5- B cells sorted from infected mice, so as to characterize the transcriptional profile of these cells and permit comparison to atMBCs in humans. Results: FCRL5+ B cells were found to have distinct transcriptional profiles from FCRL5- B cells, with approximately 400 genes exhibiting significant differences between the two groups. Additionally, about 25% of these differentially expressed genes were also differentially expressed in human atMBCs versus classical MBCs, as previously described by Sullivan et al (PLoS Pathogens 2015). Conclusions: FCRL5+ class-switched B cells are a transcriptionally distinct subset arising in P. chabaudi infection, with transcriptional similarities to human atMBCs that develop in chronic malaria settings.

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Project description:We performed NGS on Ig heavy chain V sequences amplified from RNA obtained from human tonsillar B cell subsets. From these NGS data, we reconstructed ongoing B cell responses as lineage trees, providing crucial in vivo developmental context. Each memory subset typically maintained its lineage, denoting mechanisms enforcing their phenotypes. FCRL4-FCRL5+ cells were the most represented memory subset in lineage trees, indicating robust participation in ongoing responses. Affinity maturation of both FCRL4-FCRL5+ and FCRL4+FCRL5+ atypical memory cells was stalled in chronic streptococcus infection, conceivably due to negative feedback from IgG immune complexes. We propose that FCRL4 and FCRL5 function as sensors of immune complexes, thus controlling atypical memory B cell responses.

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2020-08-04 | GSE144089 | GEO

Influenza-specific Memory B cell RNA seq

Project description:To determine the differences in gene expression between antigen-experienced memory B cells and naïve B cells, we isolated influenza-specific memory B cells expressing either IgG or IgM BCR and compared their transcriptomes to naive B cells using RNA-seq.

2021-01-25 | GSE164172 | GEO

Influenza-specific Memory B cell ATAC seq

Project description:To determine the differences in chromatin accessibility between antigen-experienced memory B cells and naïve B cells, we isolated influenza-specific memory B cells expressing either IgG or IgM BCR and compared the chromatin accessibility landscape to naive B cells using ATAC-seq.

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