Project description:Interactors of human ZUFSP in HEK-293T cells. Flp-in T-REx 293 cells with stable 3xFLAG-ZUFSP integration were assayed for ZUFSP interactors under three different conditions: i) Cells expressing wild-type ZUFSP, ii) Cells expressing catalytically inactive ZUFSP, iii) Cells expressing wild-type ZUFSP with additional treatment by an unspecific nuclease (Benzonase) during purification.
Project description:We looked at the distribution of the histone mark H3K36me3 in wild type 293T and SETD2 deleted 293T (KO) cells. We also looked at the H3K36me3 profile after rescuing the KO cells with recombinant SETD2 constructs; FL (full length), N3 (1404-2564) and N3ΔSRI/N3woSRI (1404-2468). Furthermore, we performed RNA-Seq of cells expressing the wild type or mutant/recombinant copy of SETD2 to compare their global transcriptome profile.