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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [scRNA-Seq]

ABSTRACT: A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [scRNA-Seq]

PROVIDER: PRJNA689722 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13357506_1.fastq.gz	Fastqsanger.gz
	SRR13357506_2.fastq.gz	Fastqsanger.gz

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella

Project description:A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [scRNA-Seq]

Project description:Interactions between intracellular bacteria and mononuclear phagocytes give rise to diverse cellular phenotypes that may determine the outcome of infection. Recent advances in single-cell RNA-seq (scRNA-seq) have identified multiple subsets within the mononuclear population, but implications to their function during infection remain unknown. Here, we applied microscopy, flow cytometry and scRNA-seq to survey the mononuclear niche of intracellular Salmonella Typhimurium (S.Tm) during early systemic infection in mice. We describe eclipse-like growth kinetics in the spleen, with a first phase of bacterial control mediated by tissue-resident red-pulp macrophages. A second phase involved extensive bacterial replication within a newly identified macrophage population characterized by CD9 expression. Using Nr4a1e2-/- mice we established that CD9+ macrophages originated from non-classical monocytes (NCM), and NCM-depleted mice were more resistant to S.Tm infection. Our study defines a novel host-pathogen interface, with macrophage subset-specific interactions that determines early infection dynamics and the infection outcome of the entire organism.

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq I]

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq I]

Project description:A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq I]

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella [Bulk RNA-Seq II]

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A non-classical monocyte-derived macrophage subset provides a splenic replication niche for intracellular Salmonella.

Project description:Interactions between intracellular bacteria and mononuclear phagocytes give rise to diverse cellular phenotypes that may determine the outcome of infection. Recent advances in single-cell RNA sequencing (scRNA-seq) have identified multiple subsets within the mononuclear population, but implications to their function during infection are limited. Here, we surveyed the mononuclear niche of intracellular Salmonella Typhimurium (S.Tm) during early systemic infection in mice. We described eclipse-like growth kinetics in the spleen, with a first phase of bacterial control mediated by tissue-resident red-pulp macrophages. A second phase involved extensive bacterial replication within a macrophage population characterized by CD9 expression. We demonstrated that CD9+ macrophages induced pathways for detoxificating oxidized lipids, that may be utilized by intracellular S.Tm. We established that CD9+ macrophages originated from non-classical monocytes (NCM), and NCM-depleted mice were more resistant to S.Tm infection. Our study defines macrophage subset-specific host-pathogen interactions that determine early infection dynamics and infection outcome of the entire organism.

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A Novel Non-Classical Monocyte-derived Macrophage Subset That Provides A Splenic Replication Niche For Intracellular Salmonella

Project description:This SuperSeries is composed of the SubSeries listed below.

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