Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA seq data of Pseudomonas fragi 1793 biofilms formed on aerobically stored, chilled beef

ABSTRACT: RNA seq data of Pseudomonas fragi 1793 biofilms formed on aerobically stored, chilled beef

PROVIDER: PRJNA691151 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13401167_1.fastq.gz	Fastqsanger.gz
	SRR13401167_2.fastq.gz	Fastqsanger.gz
	SRR13401168_1.fastq.gz	Fastqsanger.gz
	SRR13401168_2.fastq.gz	Fastqsanger.gz
	SRR13401169_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

RNA seq data of Pseudomonas fragi 1793 biofilms formed on aerobically stored, chilled beef

2021-01-12 | GSE164539 | GEO

chilled stored salmon

Project description:chilled stored salmon Metagenome

| PRJNA786236 | ENA

Pseudomonas fragi

Project description:Pseudomonas fragi overview

| PRJNA81643 | ENA

Pseudomonas fragi

Project description:Pseudomonas fragi Genome sequencing

| PRJNA674754 | ENA

Pseudomonas fragi

Project description:Pseudomonas fragi Genome sequencing

| PRJNA882128 | ENA

Pseudomonas fragi

Project description:Pseudomonas fragi Transcriptome or Gene expression

| PRJNA436220 | ENA

Pseudomonas fragi

Project description:Pseudomonas fragi Genome sequencing and assembly

| PRJNA380155 | ENA

Pseudomonas fragi

Project description:Pseudomonas fragi Genome sequencing and assembly

| PRJNA348113 | ENA

Transcriptional profiling of biofilms formed on chilled beef by psychrotrophic meat spoilage bacterium, <i>Pseudomonas fragi</i> 1793.

Project description:Pseudomonas fragi is the predominant bacterial species associated with spoiled aerobically stored chilled meat worldwide. It readily forms biofilms on meat under refrigerated temperature conditions used in the meat industry. Biofilm growth leads to slime development on meat which in turn becomes a major quality defect. To understand the genetic regulation that aids P. fragi to survive under chilled conditions used in the meat industry, as well to obtain an overview of the transcriptomic behavior of this organism when grown as biofilms, RNA sequencing was carried out for the main stages of the P. fragi 1793 biofilm. RNA was extracted at different stages of the biofilm cycle namely initiation, maturation and dispersal. At the same time, the biofilm growth was assessed by fluorescent staining and imaging using confocal laser scanning microscope. The results of RNA sequencing were verified by qRT-PCR using twelve genes that were most significantly up and down regulated at each stage. Differential expression analysis at biofilm maturation revealed 332 significantly upregulated genes and 37 downregulated genes relative to initiation. Differential expression analysis at biofilm dispersal reveled 658 upregulated and 275 downregulated genes relative to initiation. During biofilm maturation and dispersal, genes coding for flp family type IVb pilin, ribosome modulation factor, creatininase were the most upregulated genes while genes encoding for iron uptake systems including TonB-dependent siderophore receptor and taurine transport were significantly down regulated. The results show that protein synthesis and cellular multiplication cease after the biofilm population maximum has reached.

| S-EPMC7921472 | biostudies-literature

Pseudomonas fragi 1E26

Project description:Pseudomonas fragi 1E26 genome sequence

| PRJEB244 | ENA