Project description:Pseudomonas fragi is the predominant bacterial species associated with spoiled aerobically stored chilled meat worldwide. It readily forms biofilms on meat under refrigerated temperature conditions used in meat industry. Biofilm growth leads to slime development on meat which becomes a major quality defect. In this research, RNA sequencing was carried out for the main stages of P. fragi strain 1793 grown on aerobically stored meat, kept at 10 °C. RNA was extracted at different stages of the biofilm cycle namely initiation, maturation and dispersal. The key objectives of this study was to investigate which genes are expressed at each of these stages as well as to understand the causes of P. fragi biofilm dipersal.
Project description:St. 14 embryos were incubated in 100mM of a Jnk inhibitor (SP600125)-0,1x MBS until stage 24. Embryonic epidermis was isolated and stored immediately in chilled (4°C) Trizol. We sought to uncover all the putative targets of Jnk expresssed in the embryonic epidermis whole embryonic skin was isolated and immediately stored in chilled trizol
Project description:Salmon alphavirus (SAV) and Moritella viscosa causing respectively pancreatic disease and winter ulcer are among the most important pathogens threatening Atlantic salmon aquaculture. Fish is protected by vaccination with different rate of success. Here, responses to vaccination were assessed followed with pathogen challenges of vaccinated salmon and saline injected control.
Project description:This study investigates host-specific gene expression of the Pacific salmon lice, Lepeophtheirus salmonis oncorhynchii, while parasitizing a resistant host (Coho salmon), two susceptible hosts (Atlantic salmon, Sockeye salmon), and a population with-held hosts (starved), over 48 hrs.
Project description:<p>Animals and their associated microbiota share long evolutionary histories. However, it is not always clear how host genotype and microbiota interact to affect phenotype. We applied a hologenomic approach to explore how host-microbiota interactions shape lifetime growth and parasite infection in farmed Atlantic salmon (<em>Salmo salar</em>). Multi-omics data sets were generated from the guts of 460 salmon, 82% of which were naturally infected with an intestinal cestode. A single <em>Mycoplasma</em> bacterial strain, MAG01, dominated the gut metagenome of large, non-parasitized fish, consistent with previous studies showing high levels of <em>Mycoplasma</em> in the gut microbiota of healthy salmon. While small and/or parasitized salmon also had high abundance of MAG01, we observed increased alpha diversity in these individuals, driven by increased frequency of low-abundance <em>Vibrionaceae</em> and other <em>Mycoplasma</em> species that carried known virulence genes. Colonization by one of these cestode-associated <em>Mycoplasma</em> strains was associated with host individual genomic variation in long non-coding RNAs. Integrating the multi-omic data sets revealed coordinated changes in the salmon gut mRNA transcriptome and metabolome that correlated with shifts in the microbiota of smaller, parasitized fish. Our results suggest that the gut microbiota of small and/or parasitized fish is in a state of dysbiosis that partly depends on the host genotype, highlighting the value of using a hologenomic approach to incorporate the microbiota into the study of host-parasite dynamics.</p>
Project description:This SuperSeries is composed of the following subset Series: GSE26981: Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in skin of Atlantic salmon GSE26984: Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in spleen of Atlantic salmon Refer to individual Series
Project description:We investigate the effect of a functional feed for immunostimulation (peptidoglycan extract from bacterial cell wall with nucleotide formulation) on L. salmonis infection levels on Atlantic salmon Salmo salar, and on host and parasite gene expression profiles. Atlantic salmon smolts (~95 g) were fed a control diet, or a low or high dose immunostimulant diet, and then exposed to L. salmonis copepodids in three subsequent exposures. The transcriptome of salmon lice late in the infection attached to either the low dose diet or control diet hosts were compared using a 38K oligonucleotide microarray.
