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Transcriptome analysis of mouse follicular (FO) and marginal zone (MZ) B cells from control mice and of MZ B cells from mice overexpressing AKT-1 in B cells

ABSTRACT: Transcriptome analysis of mouse follicular (FO) and marginal zone (MZ) B cells from control mice and of MZ B cells from mice overexpressing AKT-1 in B cells

PROVIDER: PRJNA691912 | ENA |

REPOSITORIES: ENA

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miRNA expression profile of follicular (FO) and marginal zone (MZ) B cells

Project description:We have analyzed miRNA expression profile in FO and MZ B cells to identified differentially expressed miRNAs between this two subsets that could be potencially involved in the regulation of terminal B cell differentiation Spleens from CD19-Creki/+Dicerfl/+ and CD19-Creki/+Dicerfl/fl mice were collected and after erythrocyte lysis, splenocytes were stained with anti-B220, anti-CD21 and anti-CD23 antibodies. B220+CD21brightCD23+ (MZ) and B220+CD21+CD23bright (FO) B cell populations were sorted and total RNA of purified populations was extracted with TRIzol. miRNA microarray hybridations were performed on Mouse miRNA Microarray platform (Agilent Technologies).

2011-11-01 | E-GEOD-25139 | biostudies-arrayexpress

miRNA expression profile of follicular (FO) and marginal zone (MZ) B cells

2011-11-01 | GSE25139 | GEO

Gene expression profiling of spleen marginal zone B cells and spleen follicular B cells in IRF8 conditional KO mice

Project description:Conditional IRF8 KO mice (mice with a conditional allele of Irf8 crossed with CD19-Cre mice) showed increased numbers of both Gene expression data spleen marginal zone (MZ) and Gene expression data spleen follicular (FO) B cells compared to control mice. To evaluate gene expression patterns that distinguished FO or MZ B cells derived from conditional KO and control mice, we used Affymetrix GeneChip® Mouse gene 1.0 ST Array. FACS-sorted MZ and FO B cells from individual mouse were used for RNA extraction and Affyarray hybridization. There were six independent biological replications in each group - six cases of MZ B cells and FO cells in IRF8 conditional KO mice and six cases of MZ B cells and FO cells in control WT mice.

2010-12-20 | E-GEOD-24972 | biostudies-arrayexpress

Transcription profiling of mouse follicular and marginal zone B-cells

Project description:Immature B cells in spleens of mouse and human differentiate into at least two subsets of mature B cells, the follicular (FO) B cells and the marginal zone (MZ) B cells, but functions, maturation and other properties of these cells are largely unknown. To solve these questions, in this study, we performed transcriptome analyses of FO and MZ B cells sorted from spleens of normal unimmunized mice using gene chips. By comparison of gene expression profiles between FO and MZ B cells, we identified 1226 genes that are expressed higher in FO B cells than in MZ B cells. On the other hand, 1734 genes were found to be expressed higher in MZ B cells than in FO B cells. We noticed that some of differentially expressed genes have been reportedly characterized in FO and MZ B cells, suggesting the reliability of the analysis. By using FACS analyses, we confirmed that CD36, CD68, and CD49e are expressed on MZ B-cells but not on FO B-cells. These results revealed new phenotypic and functional properties of FO and MZ B cells, and set a molecular basis for further studying differentiation and functions of these mature B cells. Experiment Overall Design: one follicular B cells replicate and one marginal zone B cells replicate were analyzed.

2008-06-12 | E-GEOD-3599 | biostudies-arrayexpress

Gene expression profiling of spleen marginal zone B cells and spleen follicular B cells in IRF8 conditional KO mice

2010-12-20 | GSE24972 | GEO

Comparison of gene expression profiles between mouse follicular and marginal zone B-cells

Project description:Immature B cells in spleens of mouse and human differentiate into at least two subsets of mature B cells, the follicular (FO) B cells and the marginal zone (MZ) B cells, but functions, maturation and other properties of these cells are largely unknown. To solve these questions, in this study, we performed transcriptome analyses of FO and MZ B cells sorted from spleens of normal unimmunized mice using gene chips. By comparison of gene expression profiles between FO and MZ B cells, we identified 1226 genes that are expressed higher in FO B cells than in MZ B cells. On the other hand, 1734 genes were found to be expressed higher in MZ B cells than in FO B cells. We noticed that some of differentially expressed genes have been reportedly characterized in FO and MZ B cells, suggesting the reliability of the analysis. By using FACS analyses, we confirmed that CD36, CD68, and CD49e are expressed on MZ B-cells but not on FO B-cells. These results revealed new phenotypic and functional properties of FO and MZ B cells, and set a molecular basis for further studying differentiation and functions of these mature B cells. Keywords: Comparison of gene expression profiles between mouse follicular and marginal zone B-cells

2006-04-21 | GSE3599 | GEO

RNA-seq analysis of follicular (FO) and marginal zone (MZ) B cells, isolated from TLR9WT or TLR9-/- BALB/c mice, stimulated with TLR7 agonists (CL097, Invivogen) or left unstimulated .

Project description:Purpose: The goal of this study was to assess if increased TLR7 signaling could be seen in TLR9-/- compared to TLR9WT FO and MZ B cells. Methods : FO and MZ B cells from TLR9WT or TLR9-/- BALB/c mice were sorted using FACSaria. A minimum of 100,000 cells per B cell subsets were sorted. Sorted B cell subsets were stimulated for 4 hours at 0.25M/ml with TLR7 agonist CL097 (Invivogen) at 5µg/ml or left unstimulated. RNA was isolated using the RNeasy Plus Micro Kit (QIAGEN). Samples were sequenced on an Illumina Next-seq 2000 using a P3 200 cycle flowcell (Illumina, Inc) with 2 x 101 bp paired-end reads (20 million reads per sample). Results: There were no significant DEG (FDR > 0.05) following TLR7 stimulation between TLR9WT and TLR9-/- FO or MZ B cells.

2022-08-03 | GSE202105 | GEO

Transcriptomic analyis of donor (B6:SJL.C20) and host follicular and marginal zone B cells after adoptive transfer of follicular B cells into into lymphopenic (Rag2-/-) or replete animals (C57BL6)

Project description:The generation of marginal zone (MZ) B cells is a Notch2 dependent process. We observed that transdifferentiation of follicular (FO) B cells to an MZ-like phenotype is dependent on a lymphopenic environment. We therefore adoptively transftered congenically marked SJL.C20 (CD45.1) mature naive FO B cells into either lymphopenic Rag2-/- or intact C57BL6 (CD45.2) recipient mice and recovered and double sorted intermediate phenotype (MZ-FO) donor B cells at day 4 post transfer from Rag2-/- recipients or both MZ and FO phenotype donor B cells at day 8 post transfer from both recipient groups and performed RNA sequencing.

2022-05-01 | GSE200219 | GEO

Transcriptome analysis of mouse follicular (FO) and marginal zone (MZ) B cells from control mice and of MZ B cells from mice overexpressing AKT-1 in B cells

Project description:The purpose of this experiment is to delineate whether Akt over-expression is sufficient to induce a marginal zone-like phenotype in B cells at the molecular level. Mice expressing constitutively Akt in B cells have almost only marginal zone B cells in secondary lymphoid organs, as much as we can say through characterization of cell surface receptor by flow cytometry. These analyses were conducted in order to gain a more precise understanding of the relationship between WT marginal zone B cells and marginal zone B cells produced as a result of constitutive Akt signalling.

2024-12-01 | GSE164765 | GEO

Gene expresion profiling of two distnict splenic marginal zone B cell subpopulations, CD80high and CD80low MZ B cells

Project description:Marginal zone (MZ) B cells are innate-like B cells that not only rapidly secrete antibodies (Abs) against blood-borne pathogens but also serve Ab-independent functions such as antigen presentation and immune regulation, which may reflect their heterogeneity. Here, we discovered a subpopulation of MZ B cells that expressed higher levels of CD80, but not CD86, in naïve mice. CD80high MZ B cells revealed higher Ab-producing, proliferative, and IL-10-secreting capacities than CD80low MZ B cells. Notably, the CD80high MZ B cells survived 2 Gy whole-body irradiation, whereas CD80low MZ B cells were preferentially depleted by the irradiation and repleted in a month after the irradiation. The CD80high MZ B cells expressed higher levels of genes involved in proliferation, plasma cell differentiation, antioxidant response, and immune regulation. The CD80high MZ B cells contained autoreactive BCRs reactive to double-stranded DNA or type II collagen. Next-generation sequencing revealed more immunoglobulin heavy chains with a shorter complementarity-determining region 3 and no N-nucleotides in the CD80high MZ B cells than in CD80low MZ B cells. In summary, MZ B cells can be divided into two populations differing in CD80 expression, Ab-productive capacity, radioresistance, and B cell receptor repertoire, which may have different homeostatic functions.

2023-12-31 | GSE242393 | GEO

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