Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next generation sequencing for iPSC-derived macrophages

ABSTRACT: Next generation sequencing for iPSC-derived macrophages

PROVIDER: PRJNA694297 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13514058_1.fastq.gz	Fastqsanger.gz
	SRR13514058_2.fastq.gz	Fastqsanger.gz
	SRR13514059_1.fastq.gz	Fastqsanger.gz
	SRR13514059_2.fastq.gz	Fastqsanger.gz
	SRR13514060_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Next generation sequencing for iPSC-derived macrophages

Project description:We performed the next-generation sequencing for an isogenic WASP-KO macrophage model. Above 65 million clean reads per sample were obtained. We found the numerous RNA splicing asscoiated genes were overexpressed and thousands of mRNA events were aberrantly spliced in WASP-KO macrophages.

2022-04-18 | GSE165382 | GEO

Human iPSC-derived monocytes and macrophages

Project description:Human iPSC-derived monocytes and macrophages

| PRJNA693399 | ENA

Standardized generation of human iPSC-derived hematopoietic organoids and macrophages utilizing a benchtop bioreactor platform under fully defined conditions

Project description:There is a significant demand for intermediate-scale bioreactors in academic and industrial institutions to produce cells for various applications in drug screening and/or cell therapy. However, the application of these bioreactors in cultivating hiPSC-derived immune cells and other blood cells is noticeably lacking. To address this gap, we have developed a xeno-free and chemically defined intermediate-scale bioreactor platform, which allows for the generation of standardized human iPSC-derived hematopoietic organoids and subsequent continuous production of macrophages (iPSC-Mac).

2024-05-29 | GSE268458 | GEO

Next Generation Sequencing of SARS-CoV-2 infected lung epithelial cells derived from iPSC

Project description:Next Generation Sequencing of SARS-CoV-2 infected lung epithelial cells derived from iPSC

| PRJNA786238 | ENA

Expression data from iPSCs, iPSC-derived neurons, PBMC-derived Monocytes, PBMC-derived Macrophages, PBMC-derived induced microglia

Project description:iPSCs, iPSC-derived neurons, PBMC-derived Monocytes, PBMC-derived Macrophages, PBMC-derived induced microglia

2018-12-05 | GSE123349 | GEO

Human iPSC-derived monocytes and macrophages

Project description:Macrophages are pivotal effectors of host immunity and regulators of tissue homeostasis. Understanding of human macrophage biology has been hampered by the lack of reliable and scalable models for cellular and genetic studies. Human iPSC-derived monocytes and macrophages, as an unlimited source of subject genotype-specific cells, will undoubtedly play an important role in advancing our understanding of the role of macrophage biology and implication in human diseases. Here, we present RNA-Seq data of hiPSC-derived monocytes and GM-CSF or M-CSF macrophages generated by a fully optimized differentiation protocol as well as primary myeloid cells.

2021-05-05 | GSE165152 | GEO

Large-scale production of human iPSC-derived macrophages for drug screening

Project description:Tissue resident macrophages are key players in inflammatory processes and their activation and functionality is crucial in health and disease. Diseases associated with alterations in homeostasis or dysregulation of the innate immune system are numerous and include allergic reactions, autoimmune diseases as well as cancer. Hence, these cells are of high interest in drug development. Currently, the main sources of macrophages used in drug development are still primary cells isolated from blood or tissue, or immortalized cell lines (e.g. THP-1). Here, we describe an improved method for large-scale production of tissue resident macrophages from induced pluripotent stem cells (iPSC) in unprecedented yields. For this, iPSC-derived macrophages are thoroughly characterized to confirm their cell identity and thus their suitability for screening purposes. We demonstrate that this method to generate macrophages from iPSC overcomes the limitations of using primary cells, i.e. donor variability and limited availability of large cell numbers. Notably, the cells recapitulate key functional characteristics, including cytokine release, phagocytosis and migration. Genetic modifications can be introduced at the macrophage progenitor stage, so this methodology will facilitate the generation of reporters and gain- and loss-of-function mutants in an isogenetic background, essential assets for target validation.

2020-07-07 | GSE149377 | GEO

Next Generation Sequencing of macrophages cocultured with tumor cell

Project description:Next Generation Sequencing of macrophages cocultured with tumor cell

| PRJNA885443 | ENA

Next generation sequencing transcriptome analysis of patient-derived macrophages after SARS-CoV-2 mRNA vaccination

Project description:Next generation sequencing transcriptome analysis of patient-derived macrophages after SARS-CoV-2 mRNA vaccination

| PRJNA823920 | ENA

Next generation sequencing transcriptome analysis of patient-derived macrophages stimulated with SARS-CoV-2 protein

Project description:Next generation sequencing transcriptome analysis of patient-derived macrophages stimulated with SARS-CoV-2 protein

| PRJNA725825 | ENA