Project description:To systematically examine the effect of tumor cells on macrophages, the RAW264.7 cells after a 24-hour coculture with CT26 cells were subjected to RNA sequencing, the complete medium of CT26 cells were used as control.
Project description:To analyze the proximity-dependent gene expression in macrophages cocultured with tumor cells, we integrated QMID and RNA-seq to profile the transcriptome in THP1-derived macrophages cocultured with tumor cells.
Project description:We performed the next-generation sequencing for an isogenic WASP-KO macrophage model. Above 65 million clean reads per sample were obtained. We found the numerous RNA splicing asscoiated genes were overexpressed and thousands of mRNA events were aberrantly spliced in WASP-KO macrophages.
Project description:We have reported that intra-tumoral treatment with 1V270, a phospholipid-conjugated TLR7 agonist,induces local expansion an systemic dispersion of oligoclonal tumor-specific T cells by TCR repertoire analysis using next generation RNAseq methodology. Here, we examined whether systemic 1V270 therapy also induced oligoclonal expansion of tumor-specific T cells. Two groups of BALB/c mice (n=4/group) were i.p. treated with 1V270,a phospholipid-conjugated TLR7 agonist. One cohort of mice was i.v. injected with 4T1-GLF cells (2×104) on day 0. Another cohort did not receive i.v. tumor injection (no tumor-exposed mice). 4T1 cells were orthotopically inoculated on day 21. To examine clonal specificity of tumor-specific T cells, CD8+ cells were isolated from the spleens and the tumor infiltrating lymphocytes of secondarily challenged tumors after initial 1V270 therapy. The TCR repertoires were assessed by next generation RNA sequencing of both TCRαand TCR β genes.