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Burkholderia cenocepacia

ABSTRACT: Cystic fibrosis Burkholderia cepacia complex

PROVIDER: PRJNA694364 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	JAFIBB01.dat.gz	Other
	JAFIBB01.fasta.gz	Fasta.gz
	JAFIBB01.master.dat	Other
	JAFIBD01.dat.gz	Other

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Gene expression changes in Burkholderia cenocepacia ST32

Project description:Burkholderia cenocepacia sequence type 32 (ST32) represents one of the most globally distributed strains from Bukrholderia cepacia complex (Bcc), which infected 30% of Czech cystic fibrosis (CF) patients. The aim of this study was to compare gene expression in two pairs of ST32 clinical isolates that were subjected to cultivation in two different conditions, characteristic for chronic B. cenocepacia infection in CF patients. ST32 strain is known to be a problematic epidemic strain, which caused a serious outbreak at the Prague CF centre.

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Project description:Hfq proteins are RNA chaperones that play a critical role in post-transcription regulation of gene expression. Bacteria of the Burkholderia cepacia complex harbor two distinct and functional Hfq proteins, the Hfq and Hfq2. We have previously performed the functional analysis of Hfq and Hfq2 in the pathogen Burkholderia cenocepacia J2315. In order to examine the impacts of each RNA chaperone on the global transcriptome of B. cenocepacia J2315, we performed comparative transcriptome profile of mutants on the hfq and hfq2 genes, using as reference the wild-type strain.

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