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ABSTRACT: Nicotinamide Promotes Pancreatic Differentiation Through the Dual Inhibition of CK1 and ROCK Kinases in Human Embryonic Stem Cells

PROVIDER: PRJNA701836 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13708680_1.fastq.gz	Fastqsanger.gz
	SRR13708680_2.fastq.gz	Fastqsanger.gz
	SRR13708681_1.fastq.gz	Fastqsanger.gz
	SRR13708681_2.fastq.gz	Fastqsanger.gz
	SRR13708682_1.fastq.gz	Fastqsanger.gz

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Nicotinamide promotes cell survival and differentiation as kinase inhibitor in human pluripotent stem cells

Project description:We examined the effect of nicotinamide on cell survival and differentiation in human pluripotent stem cells. Nicotinamide inhibited the phosphorylation of myosin light chain, suppressed actomyosin contraction, and led to improved cell survival after individualization. Then we analyzed the global gene expression profile after 24 hours of nicotinamide and ROCK inhibitor treatment, and found that the gene expression profile of human embryonic stem cell (hESC) treated with nicotinamide was much different from that of ROCK inhibitor treatment. Further analysis demonstrates that nicotinamide is an inhibitor of multiple kinases, including ROCK and CK1. We demonstrated that nicotinamide affected human embryonic stem cell (hESC) pluripotency and differentiation as a selective kinase inhibitor.

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Project description:In this study, we examine the transcriptome of bovine TSCs under self-renewal that is sustained by inhibiting RhoA-Rock pathway. Using systems biology of spontaneous differentiation, we identify that TGFB1 induces differentiation of bovine TSCs. We analyse the transcriptome of TSCs under TGFB1 induced differentiation. We also analyze pathways of differentiation that are dependent and independent of RhoA-Rock signaling through analysis of the transcriptome of TSCs exposed to both TGFB1 and ROCK inhibition.

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