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Nicotinamide promotes cell survival and differentiation as kinase inhibitor in human pluripotent stem cells

ABSTRACT: We examined the effect of nicotinamide on cell survival and differentiation in human pluripotent stem cells. Nicotinamide inhibited the phosphorylation of myosin light chain, suppressed actomyosin contraction, and led to improved cell survival after individualization. Then we analyzed the global gene expression profile after 24 hours of nicotinamide and ROCK inhibitor treatment, and found that the gene expression profile of human embryonic stem cell (hESC) treated with nicotinamide was much different from that of ROCK inhibitor treatment. Further analysis demonstrates that nicotinamide is an inhibitor of multiple kinases, including ROCK and CK1. We demonstrated that nicotinamide affected human embryonic stem cell (hESC) pluripotency and differentiation as a selective kinase inhibitor.

ORGANISM(S): Homo sapiens

PROVIDER: GSE121230 | GEO | 2018/10/29

REPOSITORIES: GEO

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Nicotinamide promotes cell survival and differentiation as kinase inhibitor in human pluripotent stem cells

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