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Renal congestion exacerbates acute kidney injury in mice

ABSTRACT: Renal congestion exacerbates acute kidney injury in mice

PROVIDER: PRJNA702099 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13720976.fastq.gz	Fastqsanger.gz
	SRR13720977.fastq.gz	Fastqsanger.gz
	SRR13720978.fastq.gz	Fastqsanger.gz
	SRR13720979.fastq.gz	Fastqsanger.gz
	SRR13720980.fastq.gz	Fastqsanger.gz

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Renal congestion exacerbates acute kidney injury in mice

Project description:The number of heart failure (HF) patients is increasing. HF is frequently accompanied by kidney dysfunction and such organ failure is closely related. Recent investigations revealed that increased renal venous pressure, rather than decreased cardiac output, causes the deterioration of kidney function in HF patients; however, the underlying responsible mechanisms are unknown. We demonstrated that reduced blood flow speed in peritubular capillaries (PTCs) by renal congestion and upregulation of nuclear factor-κB (NF-κB) signaling synergistically exacerbate kidney injury. We generated a novel mouse model with unilateral renal congestion by coarctation of the inferior vena cava between renal veins. Intravital imaging highlighted the notable dilatation of PTCs and decreased renal blood flow speed in the congestive kidney. Renal damage after ischemia reperfusion injury was exacerbated in the congestive kidney and accumulation of polymorphonuclear leukocytes (PMNs) within PTCs was observed at the acute phase after injury. Pharmacological inhibition of NF-κB ameliorated renal congestion-mediated exacerbation of kidney injury. In vitro, adhesion of PMNs on the TNFα-stimulated endothelial cells was accelerated by perfusion of PMNs at a slower speed, which was cancelled by the inhibition of NF-κB signaling. Our study demonstrates the importance of slower blood flow accompanying activated NF-κB signaling in the congestive kidney in the exacerbation of renal injury. These mechanisms may explain how increased renal venous pressure in HF patients causes the deterioration of kidney dysfunction. Inhibition of NF-κB signaling may be a therapeutic candidate for the vicious cycle between heart and kidney failure with increased renal venous pressure.

2024-02-16 | GSE166838 | GEO

Glutamine attenuates renal ischemia-reperfusion injury and prevents acute kidney damage

Project description:Our study investigates how the systemic administration of glutamine modulates the transcriptomic profile of neutrophils and renal tubular epithelial cells in the kidney during acute kidney injury.

2021-11-13 | GSE188628 | GEO

Acute Kidney Injury Transcriptional Profiles: Single Cell RNA Sequencing reveals Injury induced Renal Developmental program reactivation and Lineage Infidelity in adult kidney

Project description:Acute Kidney Injury (AKI) is a rapid renal function decline associated with pronounced morbidity and mortality. Single Cell RNA Sequencing is a powerful tool allowing for examining transcriptional changes in multiple renal cell populations involved in the injury response. Our study reveals renal developmental gene re-activation and lineage infidelity in response to ischemia/reperfusion induced AKI, along with the novel genes which might serve as markers of acute kidney disease

2020-10-30 | GSE139506 | GEO

Propofol and Sevoflurane Anesthesia in Colorectal Cancer Surgery: Incidence of Acute Kidney Injury

Project description:The goal of this observational study is to compare anesthetic modalities (intravenous propofol anesthesia with sevoflurane gas anesthesia) in patients who underwent colorectal cancer resection surgery regarding the outcome of acute kidney injury. The main questions it aims to answer are: * is there a difference in acute kidney injury incidence in the two anesthetic modalities? * is there a difference in plasma creatinine between the two anesthetic modalities? * are there any patient characteristics or intraoperative factors that effect the incidence of acute kidney injury in either anesthetic modality? The study will analyze data from the CAN clinical trial database. (Cancer and Anesthesia: Survival After Radical Surgery - a Comparison Between Propofol or Sevoflurane Anesthesia, NCT01975064)

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Glutamine attenuates renal ischemia-reperfusion injury and prevents acute kidney damage

Project description:Acute kidney injury (AKI) represents a common complication in critically ill patients that is associated with an increased morbidity and mortality. Currently, no effective treatment options are available. Here, we show that glutamine significantly attenuates leukocyte recruitment and inflammatory signaling in human and murine tubular epithelial cells (TECs). In a murine AKI model induced by ischemia-reperfusion-injury (IRI) we show that glutamine causes transcriptomic and proteomic reprogramming in renal TECs and neutrophils, resulting in decreased epithelial apoptosis, neutrophil recruitment and improved mitochondrial functionality and respiration provoked by an ameliorated oxidative phosphorylation. We identify the proteins glutamine gamma glutamyltransferase 2 (Tgm2) and apoptosis signal-regulating kinase (Ask1) as the major targets of glutamine in apoptotic signaling. Increased Tgm2 expression and reduced Ask1 activation result in decreased JNK activation leading to a diminished mitochondrial intrinsic apoptosis in kidneys upon IRI-induced AKI and under hypoxia or following TNFα-treatment of TECs. Consequently, glutamine administration attenuated kidney injury in vivo during AKI progression as well as TEC viability in vitro under inflammatory and hypoxic conditions.

2023-03-11 | PXD029723 | Pride

Aortic gene expression after acute kidney injury

Project description:The risk of cardiovascular events rises after acute kidney injury. Leukocytes promote atherosclerotic plaque growth and instability. We here established a model of enhanced remote atherosclerosis after renal ischemia reperfusion injury and investigated the underlying inflammatory mechanisms.

2022-04-13 | GSE193275 | GEO

Transcriptome of mouse renal cortices without or with acute kidney injury, and without or with renal tubular epithelial cell-specific deficiency of the glucocorticoid receptor

Project description:Microarray expression data of the renal cortex of mice without or with myoglobinuric acute kidney injury, and without or with renal tubular epithelial cell-specific deficiency of the glucocorticoid receptor

2024-09-03 | GSE275996 | GEO

Gene expression in post cardiac surgery acute kidney injury

Project description:To determine the changes in intra-renal gene expression in a novel large animal model of post Cardiopulmonary Bypass (CPB) acute kidney injury, we collected renal medulla samples obtained 24hours post intervention. The transcriptional profile of the mRNA in these samples was measured with gene array technology. Pigs were subjected to 2.5 hours of general anaesthesia or 2.5 hours of CPB under general anaesthesia. Renal medulla samples were collected 24 hours post intervention.

2014-09-29 | E-GEOD-44782 | biostudies-arrayexpress

Single-cell RNA sequencing identifies response of renal lymphatic endothelial cells to acute kidney injury

Project description:Background: The inflammatory response to acute kidney injury (AKI) likely dictates future renal health. Lymphatic vessels are responsible for maintaining tissue homeostasis through transport and immunomodulatory roles. Due to the relative sparsity of lymphatic endothelial cells (LECs) in the kidney, past sequencing efforts have not characterized these cells and their response to AKI. Methods: Here we characterized murine renal LEC subpopulations by single-cell RNA sequencing and investigated their changes in cisplatin AKI 72 hours post injury. Data was processed using the Seurat package. We validated our findings by qPCR in LECs isolated from both cisplatin-injured and ischemia reperfusion injury, by immunofluorescence, and confirmation in in vitro human LECs. Results: We have identified renal LECs and their lymphatic vascular roles that have yet to be characterized in previous studies. We report unique gene changes mapped across control and cisplatin injured conditions. Following AKI, renal LECs alter genes involved in endothelial cell apoptosis and vasculogenic processes as well as immunoregulatory signaling and metabolism. Differences between injury models were also identified with renal LECs further demonstrating changed gene expression between cisplatin and ischemia reperfusion injury models, indicating the renal LEC response is both specific to where they lie in the lymphatic vasculature and the renal injury type. Conclusions: In this study, we uncover lymphatic vessel structural features of captured populations and injury-induced genetic changes. We further determine LEC gene expression is altered between injury models. How LECs respond to AKI may therefore be key in regulating future kidney disease progression.

2024-02-20 | GSE236199 | GEO

Glutamine attenuates renal ischemia-reperfusion injury and prevents acute kidney damage

Project description:Glutamine attenuates renal ischemia-reperfusion injury and prevents acute kidney damage

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