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ABSTRACT:
PROVIDER: PRJNA704216 | ENA |
REPOSITORIES: ENA
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Project description:Jujube (Ziziphus jujuba Mill.) is an economically and agriculturally significant fruit crop and is widely cultivated throughout the world. Heat stress has recently become one of the major abiotic stresses limiting plant growth and productivity. However, there are few studies on the transcriptome profiling of jujube subjected to heat stress. In this study, we analyzed the physiological and transcriptomic changes of heat-resistant jujube cultivar ‘HR’ and heat-sensitive cultivar ‘HS’ caused by high temperature stress. We statistically determined 984, 1468, 1727 and 2098 differentially expressed genes (DEGs) between ‘HR’ and ‘HS’ after 0, 1, 3, 5 d of heat stress, respectively. Gene Ontology (GO) enrichment analysis indicated that Aa great deal of heat-responsive genes were identified in these DEGs by Gene Ontology (GO) enrichment analysis. It suggests the distinct molecular mechanism of jujube response to heat stress. Furthermore, we validated the expression profiles of 12 candidates using qRT-PCR to further confirm the accuracy of the RNA-seq data. These results will advance our knowledge of the genes involved in the complex regulatory networks of heat stress and provide genetic resources for further improving the heat tolerance in jujube.
2020-10-02 | GSE136383 | GEO
Project description:We found a heat-resistant jujube cultivar in our previous study, but the molecular mecanism of heat-resistantance remained investigated. In the current study, we made this seedlings of jujube cultivar to be under heat stress (45°C) for 0, 1, 3, 5 and 7 days respectively. After checking the phenotypic and physiological features, the leaf samples (HR0, HR1, HR3, HR5 and HR7) were collected accordingly. RNA-seq transcriptome comparisons were performed, showing that 2266, 4907, 6120 and 2894 differentially expressed genes (DEGs) were identified among HR1 vs. HR0, HR3 vs. HR0, HR5 vs. HR0, and HR7 vs. HR0 respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the DEGs from these comparisons. it revealed that a series of biological processes involved in photosynthesis, protein processing in endoplasmic reticulum and metabolism, suggesting that lowering or upregulating these processes may contribute to improved heat resistance in this jujube cultivar.
2020-10-02 | GSE136047 | GEO
Project description:Transcriptome from transgenic jujube seedlings
| PRJEB81825 | ENA